Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115919_6oye ACATACCCCTGGACTTGTCTGGATTTGTCCCG(+AAAAAA)GATCCTCTCTTTCTACTTCCAGGAGGAACAC NGG 10 8 17 8 24 0 100 5 10
    240606115918_uswo GCTTGACAGTGTCACCCAGTGGTGACACTAGG(+AAAAAA)GATCCTGGTACACAGGGCACACCTTTCACTG NGG 10 8 17 8 24 0 100 5 10
    240606115915_7z64 CCTCACAGTGAAAGGTGTGCCCTGTGTACCAG(+AAAAAA)GATCCCTAGTGTCACCACTGGGTGACACTGT NGG 10 8 17 8 24 0 100 5 10
    240606115914_shmk TAGAAACATAAAACCAGATACATGCAAATGAG(+AAAAAA)GATCCTAACTATATTCTCAGCCACAGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606115913_9st6 AGAAGAGCCTCTGTGGCTGAGAATATAGTTAG(+AAAAAA)GATCCTCATTTGCATGTATCTGGTTTTATGT NGG 10 8 17 8 24 0 100 5 10
    240606115911_nafa TGGCCCAGCGTATGCACTGGCACTGGGAAGGG(+AAAAAA)GATCCAGAAGCGGTAGCTGAAGGAGAGACTG NGG 10 8 17 8 24 0 100 5 10
    240606115910_rmhm CGGGACAGTCTCTCCTTCAGCTACCGCTTCTG(+AAAAAA)GATCCCCTTCCCAGTGCCAGTGCATACGCTG NGG 10 8 17 8 24 0 100 5 10
    240606115908_nhtm GTGACGCTGGGTGCTGGAAGTAGCAGCTGAGG(+AAAAAA)GATCCTTCTTGGTCCTCAGGATTCTGGGAAT NGG 10 8 17 8 24 0 100 5 10
    240606115907_bchz TTCAAATTCCCAGAATCCTGAGGACCAAGAAG(+AAAAAA)GATCCCTCAGCTGCTACTTCCAGCACCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606115905_7fy6 TCAAGGACGCAACCTGGGGTTGCCAAGACCAG(+AAAAAA)GATCCAGACCCACGAGTGATGGGCCGAGGCA NGG 10 8 17 8 24 0 100 5 10