Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115954_94f5 CAGGTTACAGGGAAAACAACCTGGGGTACAGG(+AAAAAA)GATCCGGGAGTCCAAGAGCAAACGGTTATAG NGG 10 8 17 8 24 0 100 5 10
    240606115953_w8al ATGAGCAAAGGCAGGGACCGGGGCTTGCTAGG(+AAAAAA)GTACCAGCCCAGTTCTCCAGCCGTGAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606115951_2yxm GAGACCCACTTCACGGCTGGAGAACTGGGCTG(+AAAAAA)GTACCCTAGCAAGCCCCGGTCCCTGCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606115949_5qpj CCCCTGCCACCCAACCAGGTCTTTGGTGGGGG(+AAAAAA)GATCCCAAAGCCATTCTGGAAAGGGCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240606115947_bzr2 TTCCTCTGGAGCCCTTTCCAGAATGGCTTTGG(+AAAAAA)GATCCCCCCACCAAAGACCTGGTTGGGTGGC NGG 10 8 17 8 24 0 100 5 10
    240606115946_h02x TCCTGTGCCTGCCTTCTCTTTCTGCCTGTATG(+AAAAAA)GATCCCCAAAGGCAGTGAGTTCCTGGCTTGT NGG 10 8 17 8 24 0 100 5 10
    240606115944_96au AAAGGACAAGCCAGGAACTCACTGCCTTTGGG(+AAAAAA)GATCCATACAGGCAGAAAGAGAAGGCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606115942_0stz CATATAAGAACAAACAACCAAAATTTTCCCAG(+AAAAAA)GATCCCAGAGCCAGAGGCTACAAGAACAACT NGG 10 8 17 8 24 0 100 5 10
    240606115940_kn3y CAAAAAGTTGTTCTTGTAGCCTCTGGCTCTGG(+AAAAAA)GATCCTGGGAAAATTTTGGTTGTTTGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240606115938_knj6 GCTTCTCAGCCCCACCCTCACCCTCTGGCCAG(+AAAAAA)GATCCTACAAGGCTACATCCCCTGCTGCTTG NGG 10 8 17 8 24 0 100 5 10