Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606120028_xq4z TTCTAGCCTCAAGAGAGTGCTGGGGAGCTGTG(+AAAAAA)GTACCTGATGTTTCTGACCTGACTAAAACAC NGG 10 8 17 8 24 0 100 5 10
    240606120026_2017 CCTATAAAGTGGGTGGAGGGGAGAGAGGGCTG(+AAAAAA)GTACCCTATGGATGCTCTAAGGCCCATCATG NGG 10 8 17 8 24 0 100 5 10
    240606120024_6nxd GGAGCCATGATGGGCCTTAGAGCATCCATAGG(+AAAAAA)GTACCAGCCCTCTCTCCCCTCCACCCACTTT NGG 10 8 17 8 24 0 100 5 10
    240606120023_d391 GTTATCTGTTATCCTGATACATAAACTATTTG(+AAAAAA)GTACCTGAGAGGCTGGAAGACTACTCGTGAA NGG 10 8 17 8 24 0 100 5 10
    240606120022_1vz7 TGAAGTTCACGAGTAGTCTTCCAGCCTCTCAG(+AAAAAA)GTACCAAATAGTTTATGTATCAGGATAACAG NGG 10 8 17 8 24 0 100 5 10
    240606120021_vc0y ACTCTCTTCAGAGACACGAGAAGAGGGCATTG(+AAAAAA)GATCCCATTACAGATGGTTGTGAGTCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606120019_6tzx ACCACATGGTGACTCACAACCATCTGTAATGG(+AAAAAA)GATCCAATGCCCTCTTCTCGTGTCTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606120016_fm7q CACTCACACACGCCTGTCTGGGGAGTCCCGAG(+AAAAAA)GATCCCATGGATTCCACACGGCCAGGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240606120014_f7kv GGCCTCAGCCCCTGGCCGTGTGGAATCCATGG(+AAAAAA)GATCCTCGGGACTCCCCAGACAGGCGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240606120013_ryap CTGACCTTGGCTTGCCAATCGGTGAACTGAGG(+AAAAAA)GATCCCTTCAGAGCAGGTCCCAGCCAACCCA NGG 10 8 17 8 24 0 100 5 10