Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115937_0low TCAACCAAGCAGCAGGGGATGTAGCCTTGTAG(+AAAAAA)GATCCTGGCCAGAGGGTGAGGGTGGGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606115935_im0t CAGGCTGCTGCCCCTCTGCAGCTCTACCAGAG(+AAAAAA)GTACCCGGCCTACTCTGCTGGAAAACTAGGT NGG 10 8 17 8 24 0 100 5 10
    240606115933_nsrx CCCAGACCTAGTTTTCCAGCAGAGTAGGCCGG(+AAAAAA)GTACCTCTGGTAGAGCTGCAGAGGGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606115932_fk3j CAGTCTCCTCTACCCTCTGAACCACCTCCTCG(+AAAAAA)GATCCCATAGGCTACATTTTTTTTCCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240606115930_k5vy AATTTTGGAAGGAAAAAAAATGTAGCCTATGG(+AAAAAA)GATCCGAGGAGGTGGTTCAGAGGGTAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240606115928_l3td CATGTCATGCTCTTCAGTGTAGGGCTTCCCGG(+AAAAAA)GTACCGTGGTGCTTGAATATTATGCCATCTC NGG 10 8 17 8 24 0 100 5 10
    240606115926_1a4k GGTGGGAGATGGCATAATATTCAAGCACCACG(+AAAAAA)GTACCCGGGAAGCCCTACACTGAAGAGCATG NGG 10 8 17 8 24 0 100 5 10
    240606115925_ye8r AAGCTTCCAAGTGCTGGGGAAAGTCCAGCTAG(+AAAAAA)GATCCCATTGGTTCCTTGGCTTGATGTGCAA NGG 10 8 17 8 24 0 100 5 10
    240606115923_dg8r TCTGTTTGCACATCAAGCCAAGGAACCAATGG(+AAAAAA)GATCCTAGCTGGACTTTCCCCAGCACTTGGA NGG 10 8 17 8 24 0 100 5 10
    240606115922_8nfe AGTGTGTGTTCCTCCTGGAAGTAGAAAGAGAG(+AAAAAA)GATCCGGGACAAATCCAGACAAGTCCAGGGG NGG 10 8 17 8 24 0 100 5 10