Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606121343_1li0 TTAGAGTGGTGGGTTGGTCAGCTGGAAGACAG(+AAAAAA)GATCCAAAATGTTCCATGGAAGGCTGTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606121341_bqmz AGCCTAAAGACAGCCTTCCATGGAACATTTTG(+AAAAAA)GATCCTGTCTTCCAGCTGACCAACCCACCAC NGG 10 8 17 8 24 0 100 5 10
    240606121340_s9s2 GCTGAGGGGCTGAGTTCCCTTGAAGCAGCCAG(+AAAAAA)GTACCGGTGGCCAGCTCTCTCTTGTTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606121337_gh9r TGGACTCTAGAACAAGAGAGAGCTGGCCACCG(+AAAAAA)GTACCTGGCTGCTTCAAGGGAACTCAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240606121335_y4e7 CAGCATCACCTGCCCCTACCCAGTGCCCACAG(+AAAAAA)GTACCAGTAAACCTTAGGAACACAAAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240606121334_ywjx TGAAGCTGACTTTGTGTTCCTAAGGTTTACTG(+AAAAAA)GTACCTGTGGGCACTGGGTAGGGGCAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606121333_20ul ATTTTCTCTTTATAACCTTGGTTTTGTTGGAG(+AAAAAA)GATCCTCACCTAGAAAGTTGAAGACAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240606121331_2rkd CATTTATCTCTGTCTTCAACTTTCTAGGTGAG(+AAAAAA)GATCCTCCAACAAAACCAAGGTTATAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240606121330_omkt ATAGTAATGACCTTCTTTGAAAATAGCTGTGG(+AAAAAA)GTACCCATAAGAGCTTCTGAATGGGATTGTG NGG 10 8 17 8 24 0 100 5 10
    240606121328_9nc4 ATGCTCACAATCCCATTCAGAAGCTCTTATGG(+AAAAAA)GTACCCACAGCTATTTTCAAAGAAGGTCATT NGG 10 8 17 8 24 0 100 5 10