Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606120115_vfry TCCTTTTAGCAAATTACCATTGGGCAGAATCG(+AAAAAA)GATCCTGAAAAATCAAGATGCTAAAGCCCCT NGG 10 8 17 8 24 0 100 5 10
    240606120113_iij6 GGTGTCAGCGTGTGGATGCTGATTATGGGGTG(+AAAAAA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606120110_87kl GGATGGACCATGTAGAGACTGCCATATCCAGG(+AAAAAA)GATCCACCCCATAATCAGCATCCACACGCTG NGG 10 8 17 8 24 0 100 5 10
    240606120109_j4n6 TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+AAAAAA)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606120107_ocnc TCCTCAGAAAATTGGACATAGTACTACCGGAG(+AAAAAA)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240606120105_mfp1 CTTGTACTTTTCTTTATTCTCTACAAGACTGG(+AAAAAA)GATCCATCTTGGAAACATTAAGAATATCTAC NGG 10 8 17 8 24 0 100 5 10
    240606120104_fk7u ATCCAGTAGATATTCTTAATGTTTCCAAGATG(+AAAAAA)GATCCCAGTCTTGTAGAGAATAAAGAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240606120102_o5vo AATGTCTGCGTTTGGTGGCTGATTATGGGATG(+AAAAAA)GATCCCCGGATGGGGCAGTCTCTGGATAGTT NGG 10 8 17 8 24 0 100 5 10
    240606120100_vkyf ACTGTAACTATCCAGAGACTGCCCCATCCGGG(+AAAAAA)GATCCATCCCATAATCAGCCACCAAACGCAG NGG 10 8 17 8 24 0 100 5 10
    240606120058_0jki AAGCATGGACCAACTTAAAGGGTTTGCACTGG(+AAAAAA)GATCCTCTGTGGAGCTGAGTTCTACCACCTT NGG 10 8 17 8 24 0 100 5 10