Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606120129_ft0q ATTGATTTCCTGCTGATGTGTACCTTTGCTGG(+AAAAAA)GATCCGGAATAGTAAAAAGTGTTGGAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240606120127_gp42 CCTGACTTCCAGTTAAGATGGAAGATTAGAAG(+AAAAAA)GTACCTCTCCTTGTGACCCACTTAAGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240606120126_pp7v GCAGTTTCACCTTAAGTGGGTCACAAGGAGAG(+AAAAAA)GTACCTTCTAATCTTCCATCTTAACTGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606120124_1im6 AGCAAGGAAGGGCTAGCCACCATCTTGAGAAG(+AAAAAA)GTACCTGTTGTTCAAAACTAACTCACTCTGG NGG 10 8 17 8 24 0 100 5 10
    240606120123_p8ze ACCCCCCAGAGTGAGTTAGTTTTGAACAACAG(+AAAAAA)GTACCTTCTCAAGATGGTGGCTAGCCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606120121_jsfh TTTCCCTCAGTGATGAAGAGGCCCCACTGTGG(+AAAAAA)GTACCAACCAACCCTGATACATAAAGTCACA NGG 10 8 17 8 24 0 100 5 10
    240606120120_ery4 TTCCCTGTGACTTTATGTATCAGGGTTGGTTG(+AAAAAA)GTACCCACAGTGGGGCCTCTTCATCACTGAG NGG 10 8 17 8 24 0 100 5 10
    240606120119_jmpc ACTTCACAAGAAGCTCTCTGCTCTCTGCACTG(+AAAAAA)GTACCCAACTATTCAGCTAATCTTCACTTCC NGG 10 8 17 8 24 0 100 5 10
    240606120117_fz2r AGAGAGGAAGTGAAGATTAGCTGAATAGTTGG(+AAAAAA)GTACCAGTGCAGAGAGCAGAGAGCTTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240606120116_cl1s AGTGTAGGGGCTTTAGCATCTTGATTTTTCAG(+AAAAAA)GATCCGATTCTGCCCAATGGTAATTTGCTAA NGG 10 8 17 8 24 0 100 5 10