Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606113811_ghdn TCTACATTTTAATTTTTATGATTCCTGATGTG(+AAAAAA)GATCCATTACAAACTTCTCAGAAGTTTGGAG NGG 10 8 17 8 24 0 100 5 10
    240606113810_v8i9 ATGCACTCCAAACTTCTGAGAAGTTTGTAATG(+AAAAAA)GATCCACATCAGGAATCATAAAAATTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606113807_pzsh GGAAGAAAGGTGGTGTCCTTCCGGACTCCGGG(+AAAAAA)GATCCAGGCCTGAATATGGGAGGCACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240606113805_5r1m CTGAGCCTGGGTGCCTCCCATATTCAGGCCTG(+AAAAAA)GATCCCCGGAGTCCGGAAGGACACCACCTTT NGG 10 8 17 8 24 0 100 5 10
    240606113804_sopw AAAAATTACTAAAACCATGCAAAATGATTGAG(+AAAAAA)GATCCCAAAGGCTTTCTTAATGTACACTAGT NGG 10 8 17 8 24 0 100 5 10
    240606113802_fk65 TAGATACTAGTGTACATTAAGAAAGCCTTTGG(+AAAAAA)GATCCTCAATCATTTTGCATGGTTTTAGTAA NGG 10 8 17 8 24 0 100 5 10
    240606113801_rwsu TCTTGGATGGACTTCTAAAATATATGTTGATG(+AAAAAA)GATCCCTTTTTTATTTTGTGTTTTTTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240606113759_2sj6 ACTATATTAAAAAAAACACAAAATAAAAAAGG(+AAAAAA)GATCCATCAACATATATTTTAGAAGTCCATC NGG 10 8 17 8 24 0 100 5 10
    240606113758_cqv8 ACACCGCCACGCTGTTTTGTTTTGTTTCTTTG(+AAAAAA)GATCCTGGCCTGGCATGTCGATAGTCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606113756_upol CTTATCTTCTGACTATCGACATGCCAGGCCAG(+AAAAAA)GATCCAAAGAAACAAAACAAAACAGCGTGGC NGG 10 8 17 8 24 0 100 5 10