Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606113708_d01p GAAATAAAATCAAAACTTACATATATTCTTAG(+AAAAAA)GATCCTGCATGGAGGTGCTTTTCTCTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606113707_830p CCCCACCCAAAGAGAAAAGCACCTCCATGCAG(+AAAAAA)GATCCTAAGAATATATGTAAGTTTTGATTTT NGG 10 8 17 8 24 0 100 5 10
    240606113706_9iho CCAGTGGCATCATGTAAGGTGATACACATATG(+AAAAAA)GATCCTTGTTCTGTTTTACAAGTATATCACC NGG 10 8 17 8 24 0 100 5 10
    240606113704_bjgt TAAATGGTGATATACTTGTAAAACAGAACAAG(+AAAAAA)GATCCATATGTGTATCACCTTACATGATGCC NGG 10 8 17 8 24 0 100 5 10
    240606113703_rqhr TCCAGGGAATTAGCCTCCTCTGGTGACTATGG(+AAAAAA)GTACCAGGTACACATAAGGTGCACAAACATA NGG 10 8 17 8 24 0 100 5 10
    240606113701_39ue TCATGTATGTTTGTGCACCTTATGTGTACCTG(+AAAAAA)GTACCCATAGTCACCAGAGGAGGCTAATTCC NGG 10 8 17 8 24 0 100 5 10
    240606113659_flxs CTTCAGTGAAGCATGCCAGAGGCAGTGAACGG(+AAAAAA)GTACCATAAGTGGCTGCTGGGATATACCAGG NGG 10 8 17 8 24 0 100 5 10
    240606113658_mqxq GCCTACCTGGTATATCCCAGCAGCCACTTATG(+AAAAAA)GTACCCGTTCACTGCCTCTGGCATGCTTCAC NGG 10 8 17 8 24 0 100 5 10
    240606113654_4cwi GAGGGTTTTCAGTTGTGACAGCCCCACCCCCG(+AAAAAA)GTACCATCTCTCTCCTCAATGTAGTCATGCA NGG 10 8 17 8 24 0 100 5 10
    240606113653_2dzc GTGCTTGCATGACTACATTGAGGAGAGAGATG(+AAAAAA)GTACCGGGGGTGGGGCTGTCACAACTGAAAA NGG 10 8 17 8 24 0 100 5 10