Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606113635_78vc TTCTTGGCATGAGCCTATCCTTATCGCCCTTG(+AAAAAA)GATCCCAGTTTAACAAGACCTTTTTCTAAAG NGG 10 8 17 8 24 0 100 5 10
    240606113633_vahb ATTGTCTTTAGAAAAAGGTCTTGTTAAACTGG(+AAAAAA)GATCCAAGGGCGATAAGGATAGGCTCATGCC NGG 10 8 17 8 24 0 100 5 10
    240606113632_yllb TGCATCTTCATCTTCATCACGACACCAGACAG(+AAAAAA)GATCCAATCTTGGATGGAGACATTACCAGTG NGG 10 8 17 8 24 0 100 5 10
    240606113631_73di CCCTTCACTGGTAATGTCTCCATCCAAGATTG(+AAAAAA)GATCCTGTCTGGTGTCGTGATGAAGATGAAG NGG 10 8 17 8 24 0 100 5 10
    240606113629_gkfj GTTGTTTGGTTTGTTTATTTCTGTGGTTCTGG(+AAAAAA)GATCCAGCCCAGGGCCTTACATGTGCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240606113627_pg4r TATTTGTCTAGCACATGTAAGGCCCTGGGCTG(+AAAAAA)GATCCCAGAACCACAGAAATAAACAAACCAA NGG 10 8 17 8 24 0 100 5 10
    240606113625_0vzv GTTACAGCAGGGATTTGACAAAACACAGGGTG(+AAAAAA)GATCCATGCAGTTTGGAAACTTAACTTTGAA NGG 10 8 17 8 24 0 100 5 10
    240606113624_bzo5 GATCGTTCAAAGTTAAGTTTCCAAACTGCATG(+AAAAAA)GATCCACCCTGTGTTTTGTCAAATCCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240606113622_hp9j TTTCTAAGCCACACAAGATGGGGTAAAAAGGG(+AAAAAA)GTACCAACACCTCAATGGCAGTTAGACTTCC NGG 10 8 17 8 24 0 100 5 10
    240606113621_dgeo TGAGTGGAAGTCTAACTGCCATTGAGGTGTTG(+AAAAAA)GTACCCCTTTTTACCCCATCTTGTGTGGCTT NGG 10 8 17 8 24 0 100 5 10