Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606113549_gofa CCATTGTTCCAAGGCTGCACATGGCTTGGAAG(+AAAAAA)GATCCTGTTTGAGTGTATGCTTCCTCACTCA NGG 10 8 17 8 24 0 100 5 10
    240606113547_0tqs GGAGCTGAGTGAGGAAGCATACACTCAAACAG(+AAAAAA)GATCCTTCCAAGCCATGTGCAGCCTTGGAAC NGG 10 8 17 8 24 0 100 5 10
    240606113546_gy7f ACTAGGAACAACTAGTTGAGCAAGTCTTTAAG(+AAAAAA)GTACCGATTGTCAAAATACATCACTAAGACA NGG 10 8 17 8 24 0 100 5 10
    240606113545_ms0b TAATTTGTCTTAGTGATGTATTTTGACAATCG(+AAAAAA)GTACCTTAAAGACTTGCTCAACTAGTTGTTC NGG 10 8 17 8 24 0 100 5 10
    240606113544_u9vk AATGCCTTCTTCAGTTTACCAGCAGTGGTGTG(+AAAAAA)GTACCAGTGAGGTATTTCAGGTAACGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606113542_dytc AAGAGAGAGACGTTACCTGAAATACCTCACTG(+AAAAAA)GTACCACACCACTGCTGGTAAACTGAAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606113540_kwxr TTGGTACTATGATATCAAAATCAGAGGGAGGG(+AAAAAA)GATCCAGCTACCAGAACGGGAGTGGGTTCAG NGG 10 8 17 8 24 0 100 5 10
    240606113539_6bec CCTCCCTGAACCCACTCCCGTTCTGGTAGCTG(+AAAAAA)GATCCCCTCCCTCTGATTTTGATATCATAGT NGG 10 8 17 8 24 0 100 5 10
    240606113537_f190 TTGGACAGCCAGGGCTACACAGAGAGATCCTG(+AAAAAA)GATCCCGTCTTCCAAAAAAAAAAAAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606113536_1niz CTTTCTTTCTTTTTTTTTTTTTTGGAAGACGG(+AAAAAA)GATCCAGGATCTCTCTGTGTAGCCCTGGCTG NGG 10 8 17 8 24 0 100 5 10