Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606113447_2gj9 CAAACATTGTTTACCTTGTGTTTCACCCTTGG(+AAAAAA)GTACCACCCATCTACAAGTAGAAATCAGTAC NGG 10 8 17 8 24 0 100 5 10
    240606113445_qzcm GCCCAGTACTGATTTCTACTTGTAGATGGGTG(+AAAAAA)GTACCCAAGGGTGAAACACAAGGTAAACAAT NGG 10 8 17 8 24 0 100 5 10
    240606113444_d0eh GGTGCTGCCCATGATACGGCACAGCCACAGTG(+AAAAAA)GTACCAGCATTGCTGCCATGTTGGCCATCCA NGG 10 8 17 8 24 0 100 5 10
    240606113443_7g84 CTGGCTGGATGGCCAACATGGCAGCAATGCTG(+AAAAAA)GTACCACTGTGGCTGTGCCGTATCATGGGCA NGG 10 8 17 8 24 0 100 5 10
    240606113441_rs0c AGCTTCCTCTCTGTAGTTCCCTGAGGTGGGAG(+AAAAAA)GTACCCACTTTGCCATGCATAACTCATCTCT NGG 10 8 17 8 24 0 100 5 10
    240606113439_k9dc GGAAGAGAGATGAGTTATGCATGGCAAAGTGG(+AAAAAA)GTACCTCCCACCTCAGGGAACTACAGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240606113438_jzed CCTGTGCCCACTGGAGCCAGAAGAGGGCATTG(+AAAAAA)GATCCTGAAACTGAAGTTACAGATGTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240606113437_cgm6 GTGGCTCCCAACATCTGTAACTTCAGTTTCAG(+AAAAAA)GATCCAATGCCCTCTTCTGGCTCCAGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240606113435_hwet AGGGCTGAGAAGGTAGGAGCAGTACTGCAGGG(+AAAAAA)GATCCTAAGCCAGAGGTCTGGTTAATTTACA NGG 10 8 17 8 24 0 100 5 10
    240606113434_f7i5 TATGGTGTAAATTAACCAGACCTCTGGCTTAG(+AAAAAA)GATCCCCTGCAGTACTGCTCCTACCTTCTCA NGG 10 8 17 8 24 0 100 5 10