Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606115207_so3r CTCAAGTACTCCCTCCTCTATAGTTCACTAAG(+AAAAAA)GATCCAGGGACTGGTTTAACCCTTACTTAGT NGG 10 8 17 8 24 0 100 5 10
    240606115205_vaei TTCCTCATGGTCTCCTCCTACATCAAGCCAGG(+AAAAAA)GTACCCTCAATCCCCACTTTTGGCTCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240606115204_tp2k CTGGGCAGAAGAGCCAAAAGTGGGGATTGAGG(+AAAAAA)GTACCCTGGCTTGATGTAGGAGGAGACCATG NGG 10 8 17 8 24 0 100 5 10
    240606115202_hahz GTTGGTTTTGTTGTTTGTTTTTTCCGACGTTG(+AAAAAA)GTACCTTGGAAAGGTTGTGTATTTTAAAGTC NGG 10 8 17 8 24 0 100 5 10
    240606115200_b1jv TTAGTGACTTTAAAATACACAACCTTTCCAAG(+AAAAAA)GTACCAACGTCGGAAAAAACAAACAACAAAA NGG 10 8 17 8 24 0 100 5 10
    240606115159_o13r GCCCTCGTCAGAACAGAGCCCCACCACTGCTG(+AAAAAA)GTACCCGCATGGATGATGTGTGTGAATTGCC NGG 10 8 17 8 24 0 100 5 10
    240606115158_y5bi TACTTGGCAATTCACACACATCATCCATGCGG(+AAAAAA)GTACCAGCAGTGGTGGGGCTCTGTTCTGACG NGG 10 8 17 8 24 0 100 5 10
    240606115156_7shp AAAGTGTACATCAAGACCAACAACATCCTGTG(+AAAAAA)GTACCGCAGGATCCAGGAAAGCAGCCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240606115154_y4yz TGTCCACACAGGCTGCTTTCCTGGATCCTGCG(+AAAAAA)GTACCACAGGATGTTGTTGGTCTTGATGTAC NGG 10 8 17 8 24 0 100 5 10
    240606115152_alox ATCAAGACCAACAACATCCTGTGGTACCGCAG(+AAAAAA)GATCCAGGAAAGCAGCCTGTGTGGACACACA NGG 10 8 17 8 24 0 100 5 10