Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606113346_7gfm GGTCGGACTTGTGACTTAGGCTTGCAGCCTTG(+AAAAAA)GATCCTGGGGTTTGCACTGATATGGTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240606113343_3ima GGATGACAGAACCATATCAGTGCAAACCCCAG(+AAAAAA)GATCCAAGGCTGCAAGCCTAAGTCACAAGTC NGG 10 8 17 8 24 0 100 5 10
    240606113340_9af9 CATAGCATTCACCATGATCATATGCCCCTCGG(+AAAAAA)GATCCTGAGAGAAGAGAGAGGCTTGTACTGA NGG 10 8 17 8 24 0 100 5 10
    240606113339_l099 ACCCCTCAGTACAAGCCTCTCTCTTCTCTCAG(+AAAAAA)GATCCCGAGGGGCATATGATCATGGTGAATG NGG 10 8 17 8 24 0 100 5 10
    240606113338_fzs6 GATAGACATTATCTCCCCCCCCCCTTTTTCAG(+AAAAAA)GTACCTGTAATTTGTGTCTGAGAGGCAGGGT NGG 10 8 17 8 24 0 100 5 10
    240606113336_677h TTCCCACCCTGCCTCTCAGACACAAATTACAG(+AAAAAA)GTACCTGAAAAAGGGGGGGGGGAGATAATGT NGG 10 8 17 8 24 0 100 5 10
    240606113335_ja5k GGTGTCATCGTTTGGAGGCTGATTATGGGATG(+AAAAAA)GATCCCCGGGTATGGCAGTCTCAGAATACTT NGG 10 8 17 8 24 0 100 5 10
    240606113332_jddo GATAAAAGTATTCTGAGACTGCCATACCCGGG(+AAAAAA)GATCCATCCCATAATCAGCCTCCAAACGATG NGG 10 8 17 8 24 0 100 5 10
    240606113330_e2uc AGGTATTCCCCGGTCCCTACAGCTAATCCATG(+AAAAAA)GATCCCTGAAAAGGGACTGCAGGCAGGACAG NGG 10 8 17 8 24 0 100 5 10
    240606113329_8p83 CAGTACTGTCCTGCCTGCAGTCCCTTTTCAGG(+AAAAAA)GATCCATGGATTAGCTGTAGGGACCGGGGAA NGG 10 8 17 8 24 0 100 5 10