Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606113312_00oh ATCATGGAAGGCATGCCAAGTCCACCCGCTGG(+AAAAAA)GATCCAGCGGGGCACAGTACAGTTCCTCCGG NGG 10 8 17 8 24 0 100 5 10
    240606113310_clm1 AGGAGCCGGAGGAACTGTACTGTGCCCCGCTG(+AAAAAA)GATCCCAGCGGGTGGACTTGGCATGCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606113309_wk89 ACTAGGCAGAGCAAACATCAGGAAGTGGGAAG(+AAAAAA)GTACCGATATGAAATTAAGAGAAAGGCCGAC NGG 10 8 17 8 24 0 100 5 10
    240606113307_zjve TTTGAGTCGGCCTTTCTCTTAATTTCATATCG(+AAAAAA)GTACCTTCCCACTTCCTGATGTTTGCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240606113306_fgkv CCCTTGGTCCCTAATCAGTGCCTGCTGGCGTG(+AAAAAA)GATCCCAGAGGAGCCTCTGTACATGCCATCC NGG 10 8 17 8 24 0 100 5 10
    240606113305_h4kg ACTCTGGATGGCATGTACAGAGGCTCCTCTGG(+AAAAAA)GATCCACGCCAGCAGGCACTGATTAGGGACC NGG 10 8 17 8 24 0 100 5 10
    240606113303_9sb2 CATGCAATAAATGACCATTACAGGTTTCAAAG(+AAAAAA)GTACCCTAGGTTTTCTAGGTGAGAGTTCACA NGG 10 8 17 8 24 0 100 5 10
    240606113301_mc7j GTGGGTGTGAACTCTCACCTAGAAAACCTAGG(+AAAAAA)GTACCTTTGAAACCTGTAATGGTCATTTATT NGG 10 8 17 8 24 0 100 5 10
    240606113300_aq8e GGGCTGAGTGGAGACCCAGTTGGAGGAGGCTG(+AAAAAA)GATCCTTTGTTAGGAAGGCAAAGGCCCGGGG NGG 10 8 17 8 24 0 100 5 10
    240606113258_lhnu AGGTTCCCCGGGCCTTTGCCTTCCTAACAAAG(+AAAAAA)GATCCAGCCTCCTCCAACTGGGTCTCCACTC NGG 10 8 17 8 24 0 100 5 10