Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606113242_eoe4 TATTATAGCTACTGCTGCCGCTACTGTTGTTG(+AAAAAA)GTACCACCTCAGATACATAGAGGGACACATA NGG 10 8 17 8 24 0 100 5 10
    240606113240_valv TGATTTATGTGTCCCTCTATGTATCTGAGGTG(+AAAAAA)GTACCAACAACAGTAGCGGCAGCAGTAGCTA NGG 10 8 17 8 24 0 100 5 10
    240606113239_6491 CGTCTGCTTCCCTCTCTCCTCGTGCTAAGCTG(+AAAAAA)GATCCGTGTCCTTTTCTTTTTAACGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240606113238_04rg TCACTTGAACTCGTTAAAAAGAAAAGGACACG(+AAAAAA)GATCCAGCTTAGCACGAGGAGAGAGGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240606113237_rvpd GCTCACCCGCAAGGGGTTCCAGAACAAATCTG(+AAAAAA)GTACCCACGAGGACAAGCCAAACCAAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240606113235_crb7 CCTGATCCACTTGGTTTGGCTTGTCCTCGTGG(+AAAAAA)GTACCAGATTTGTTCTGGAACCCCTTGCGGG NGG 10 8 17 8 24 0 100 5 10
    240606113234_3e4x AAGAGAAGTAGGAGGTTGACTGACCAACTTGG(+AAAAAA)GTACCATAGGATTTTGTTTATTGCATAGGTC NGG 10 8 17 8 24 0 100 5 10
    240606113232_gvde TAGATGACCTATGCAATAAACAAAATCCTATG(+AAAAAA)GTACCCAAGTTGGTCAGTCAACCTCCTACTT NGG 10 8 17 8 24 0 100 5 10
    240606113230_v0fj GCTATGCACCCCTCTCCCTGGCACTGACCCTG(+AAAAAA)GATCCCCATCTTCTGTACCCAGTGACTAGAC NGG 10 8 17 8 24 0 100 5 10
    240606113227_i3na CAGTCGTCTAGTCACTGGGTACAGAAGATGGG(+AAAAAA)GATCCAGGGTCAGTGCCAGGGAGAGGGGTGC NGG 10 8 17 8 24 0 100 5 10