Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606113432_z0fk TCCTCTGAACTCTCTCATCTACCTGAAAACTG(+AAAAAA)GATCCACAGAACAGTTAACTTTGATCCCACC NGG 10 8 17 8 24 0 100 5 10
    240606113431_51uo CTCAGGGTGGGATCAAAGTTAACTGTTCTGTG(+AAAAAA)GATCCAGTTTTCAGGTAGATGAGAGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240606113430_r1sw ACCAGTGTTCTTTATACACTCTATTCCAGGTG(+AAAAAA)GATCCTTTGACATTTACGTGGTTTGGTTGGT NGG 10 8 17 8 24 0 100 5 10
    240606113428_p6qv ATGCCACCAACCAAACCACGTAAATGTCAAAG(+AAAAAA)GATCCACCTGGAATAGAGTGTATAAAGAACA NGG 10 8 17 8 24 0 100 5 10
    240606113427_zcuz AGTCCAAAAGTCACTCTGAGATCCAAGCTTCG(+AAAAAA)GATCCCCCAGACAGTGGCATCACCTCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240606113425_pha1 ATGCAAGACAGAGGTGATGCCACTGTCTGGGG(+AAAAAA)GATCCGAAGCTTGGATCTCAGAGTGACTTTT NGG 10 8 17 8 24 0 100 5 10
    240606113424_udln TTTTTCTTGAGCAGTAAACTGCAATTTTCTAG(+AAAAAA)GATCCTTTGCTGTGGACACATGCCCATCATC NGG 10 8 17 8 24 0 100 5 10
    240606113423_gkf6 TGATGGATGATGGGCATGTGTCCACAGCAAAG(+AAAAAA)GATCCTAGAAAATTGCAGTTTACTGCTCAAG NGG 10 8 17 8 24 0 100 5 10
    240606113421_1tol GAATGTCAACTGTTCATTTTGTTGCTGCATAG(+AAAAAA)GTACCATTGAGGGGGCACACTGGAATGTGCT NGG 10 8 17 8 24 0 100 5 10
    240606113420_61rq GGACAAGCACATTCCAGTGTGCCCCCTCAATG(+AAAAAA)GTACCTATGCAGCAACAAAATGAACAGTTGA NGG 10 8 17 8 24 0 100 5 10