Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231917_apeg CCAAATAAAAGAACAGGGGAAATTATGGGCAG(+CAGTGT)GATCCTAGGCAATAACTAAAGGTATAGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605231915_g4fu TTAGACAACCTATACCTTTAGTTATTGCCTAG(+CAGTGT)GATCCTGCCCATAATTTCCCCTGTTCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605231913_4nvg CTGAAGAATGTCACCACCTAGGACATGCCTTG(+CAGTGT)GTACCTGAAGTTTTCATAAAGGTTTCCATAA NGG 10 8 17 8 24 0 100 5 10
    240605231912_y8p3 TTCATTTATGGAAACCTTTATGAAAACTTCAG(+CAGTGT)GTACCAAGGCATGTCCTAGGTGGTGACATTC NGG 10 8 17 8 24 0 100 5 10
    240605231910_jplt GGTGTCAGCATTTGGAAGCTGATTATAGGATG(+CAGTGT)GATCCCTGCATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605231909_5dz6 AGATGGACCATCTAGTGATTGCCATATGCAGG(+CAGTGT)GATCCATCCTATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240605231908_75mp TACTGTGATTTTCATAACCCGTTGCCTTTTTG(+CAGTGT)GTACCAGAGCTACGTGGTTTGAATCCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605231907_jxx7 CATGTAGCCAGGATTCAAACCACGTAGCTCTG(+CAGTGT)GTACCAAAAAGGCAACGGGTTATGAAAATCA NGG 10 8 17 8 24 0 100 5 10
    240605231904_b2rv GGAGTCAAGGACTATAGTCAGGAGACCCCCAG(+CAGTGT)GTACCTGGTTCAGAGCAGTCCGGGATGATAG NGG 10 8 17 8 24 0 100 5 10
    240605231902_dooq TCGTCCTATCATCCCGGACTGCTCTGAACCAG(+CAGTGT)GTACCTGGGGGTCTCCTGACTATAGTCCTTG NGG 10 8 17 8 24 0 100 5 10