Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231811_ewzg GAAAAGGAGGTTCAGCGACAGACCCAAAGTAG(+CAGTGT)GATCCAGATCAAGGAGATGTCCCAAGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605231810_azal AGTGTGAGGCCTTGGGACATCTCCTTGATCTG(+CAGTGT)GATCCTACTTTGGGTCTGTCGCTGAACCTCC NGG 10 8 17 8 24 0 100 5 10
    240605231808_j90d TCCTCAGAAAATTGGACATAGTACTACCGGAG(+CAGTGT)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240605231806_5ap3 TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+CAGTGT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605231804_ep5c GGATGGACCATGTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605231802_4kva GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+CAGTGT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605231801_0rxd CTTTAAATGATGTGGAAATGGACAGACTCTTG(+CAGTGT)GATCCCATGGCTTAATTTTGAACAGGGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605231758_sx5w GCCTAGTTCCCCTGTTCAAAATTAAGCCATGG(+CAGTGT)GATCCAAGAGTCTGTCCATTTCCACATCATT NGG 10 8 17 8 24 0 100 5 10
    240605231757_u7z0 GAGCCCATCCCTGGCACTGCCTGGGGGGCTAG(+CAGTGT)GATCCAGAGGGTGGATAGCCCAGAGGCCTAG NGG 10 8 17 8 24 0 100 5 10
    240605231755_wba6 CTATCCTAGGCCTCTGGGCTATCCACCCTCTG(+CAGTGT)GATCCTAGCCCCCCAGGCAGTGCCAGGGATG NGG 10 8 17 8 24 0 100 5 10