Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231753_yly1 TTCTTAACACTGACCATATTCGGGGGTGGGGG(+CAGTGT)GATCCAAAAATTACAAACCATTTACTAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605231752_e2tc ATTTTCAACTAGTAAATGGTTTGTAATTTTTG(+CAGTGT)GATCCCCCCACCCCCGAATATGGTCAGTGTT NGG 10 8 17 8 24 0 100 5 10
    240605231751_h048 TTGTTGTTTTTTTTTTTTCTTACTAAAAGTGG(+CAGTGT)GATCCTTTTAGGCAGGATTTTATTCTGTAGC NGG 10 8 17 8 24 0 100 5 10
    240605231749_n38f CTTTGGCTACAGAATAAAATCCTGCCTAAAAG(+CAGTGT)GATCCCACTTTTAGTAAGAAAAAAAAAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605231748_xmij TAACCTCAGCCCTGAGTTCAGGGAGACAGGTG(+CAGTGT)GATCCCATGGACTCACTGGCTGGATAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605231745_vcut TTGATTAGCTTATCCAGCCAGTGAGTCCATGG(+CAGTGT)GATCCACCTGTCTCCCTGAACTCAGGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605231744_ohyt TGGTATGAGGCAAAGCCTCTTGAACACAAATG(+CAGTGT)GTACCCTCTTCATAATGGCTTTATTCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605231743_4bnk GGATCCAAAGGAATAAAGCCATTATGAAGAGG(+CAGTGT)GTACCATTTGTGTTCAAGAGGCTTTGCCTCA NGG 10 8 17 8 24 0 100 5 10
    240605231741_yx4s GGTACCCTCTTCATAATGGCTTTATTCCTTTG(+CAGTGT)GATCCTATTAAAGATTCCCTGCAGCTTGTAC NGG 10 8 17 8 24 0 100 5 10
    240605231739_ua23 CACTGGTACAAGCTGCAGGGAATCTTTAATAG(+CAGTGT)GATCCAAAGGAATAAAGCCATTATGAAGAGG NGG 10 8 17 8 24 0 100 5 10