Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231706_wdyt CTTGCTCTCCAGGAGCTCACAGTTCAAGCTGG(+CAGTGT)GATCCAGATGAGCAAACATGATCATCACACA NGG 10 8 17 8 24 0 100 5 10
    240605231705_7wx2 TATACTGTGTGATGATCATGTTTGCTCATCTG(+CAGTGT)GATCCCAGCTTGAACTGTGAGCTCCTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605231703_2h3a GGTGTCAGAGTTTGGATGCTGATTATGGGGTG(+CAGTGT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605231701_ecsp GGATGGACCATGTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCATCCAAACTCTG NGG 10 8 17 8 24 0 100 5 10
    240605231659_3e9h ACGGACTGCTAAATTGGGTCCTATAGATCGAG(+CAGTGT)GTACCATAATTAAGATATTTTAATAAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605231658_4my1 ATGTTGAGCCTTATTAAAATATCTTAATTATG(+CAGTGT)GTACCTCGATCTATAGGACCCAATTTAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605231656_tgy0 TATCTCCATGTACTCATGTGAATATAGCCATG(+CAGTGT)GATCCTATAGTGAGCTATATCCTTACTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605231655_f6yy AAGGTAAAAAGTAAGGATATAGCTCACTATAG(+CAGTGT)GATCCATGGCTATATTCACATGAGTACATGG NGG 10 8 17 8 24 0 100 5 10
    240605231653_szrb GCTGATGTGCAGGGGGAAGTAGGATGGCAGGG(+CAGTGT)GTACCTAGAGCCCCCTTGATAAATTTCAGAT NGG 10 8 17 8 24 0 100 5 10
    240605231652_j6ca AGGACATCTGAAATTTATCAAGGGGGCTCTAG(+CAGTGT)GTACCCCTGCCATCCTACTTCCCCCTGCACA NGG 10 8 17 8 24 0 100 5 10