Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231637_b59y TTTCTGGCTCCTAAACGCCTTTGAGCTTGTTG(+CAGTGT)GTACCCAGAGAAATAAACTAAAATTGTATGA NGG 10 8 17 8 24 0 100 5 10
    240605231636_wiy5 TTTTTTCATACAATTTTAGTTTATTTCTCTGG(+CAGTGT)GTACCAACAAGCTCAAAGGCGTTTAGGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605231634_h7p1 AGGTTTTATTGTTCTTAAACAAGGTCAGCCAG(+CAGTGT)GTACCAGATTCTAAAAAGAACAATTGCCCCC NGG 10 8 17 8 24 0 100 5 10
    240605231632_3l0x AGGGGGGGGGCAATTGTTCTTTTTAGAATCTG(+CAGTGT)GTACCTGGCTGACCTTGTTTAAGAACAATAA NGG 10 8 17 8 24 0 100 5 10
    240605231629_dduo GAGTAAGCTCCAGCCCCCAGCACCTTCCCTGG(+CAGTGT)GTACCAAGAGTCATCCGAGGGGAGGAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605231628_qcva CTTCCCCAGCTCCTCCCCTCGGATGACTCTTG(+CAGTGT)GTACCCAGGGAAGGTGCTGGGGGCTGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605231625_s0d2 CCCATTCGAGCAAATGGCCTGATGGTCCCCAG(+CAGTGT)GATCCTTGGTGGAAGTAGGCAGTGTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605231623_471d TGACTTCCTGCACACTGCCTACTTCCACCAAG(+CAGTGT)GATCCTGGGGACCATCAGGCCATTTGCTCGA NGG 10 8 17 8 24 0 100 5 10
    240605231621_h9lb TCTGGTGGGGGGCTATGCTGGAGATGAAAGGG(+CAGTGT)GATCCCAGCATGAGCCTGGAGGTCCCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240605231619_qk7d TCCACTGCCAGGGACCTCCAGGCTCATGCTGG(+CAGTGT)GATCCCCTTTCATCTCCAGCATAGCCCCCCA NGG 10 8 17 8 24 0 100 5 10