Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231602_xeqn CTGCAAAGGGAAGGAGTACAGGAAGCGCCATG(+CAGTGT)GATCCTACAGTGTTAGTTTGTCAGCTAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605231601_896k CATGTTGGCTAGCTGACAAACTAACACTGTAG(+CAGTGT)GATCCATGGCGCTTCCTGTACTCCTTCCCTT NGG 10 8 17 8 24 0 100 5 10
    240605231600_y69v CAATACAGTAAGTTCAATTGAGAGGCCAAACG(+CAGTGT)GATCCAAGGAAGATAAAAATTAAATACTTAA NGG 10 8 17 8 24 0 100 5 10
    240605231558_ckhe TTAATTTAAGTATTTAATTTTTATCTTCCTTG(+CAGTGT)GATCCGTTTGGCCTCTCAATTGAACTTACTG NGG 10 8 17 8 24 0 100 5 10
    240605231556_thxj CTGGGTCTTCCCAGAGCAGACATTTTCACCAG(+CAGTGT)GTACCTTACCCAAAACAAGTGAGGTGATCTC NGG 10 8 17 8 24 0 100 5 10
    240605231555_a9kp CCTCTGAGATCACCTCACTTGTTTTGGGTAAG(+CAGTGT)GTACCTGGTGAAAATGTCTGCTCTGGGAAGA NGG 10 8 17 8 24 0 100 5 10
    240605231553_k0cn AATCCAAATCTTCATTCATTTCTGGCACAAAG(+CAGTGT)GTACCAGGAAATGATGTACTATCAGGAAATC NGG 10 8 17 8 24 0 100 5 10
    240605231551_hq9b TGTGTGATTTCCTGATAGTACATCATTTCCTG(+CAGTGT)GTACCTTTGTGCCAGAAATGAATGAAGATTT NGG 10 8 17 8 24 0 100 5 10
    240605231549_7w21 AGATTTAAAAACACCAAAAGCTGCTATCCTTG(+CAGTGT)GTACCAAGAACTGTGCATATGTGTTCAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605231548_5grh GTCTATTTCTGAACACATATGCACAGTTCTTG(+CAGTGT)GTACCAAGGATAGCAGCTTTTGGTGTTTTTA NGG 10 8 17 8 24 0 100 5 10