Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231501_tw87 GGGATTCAGTGACCTTTCCTGGTTCCCACAGG(+CAGTGT)GTACCTGAACTCATGTGTATAGATGCACACA NGG 10 8 17 8 24 0 100 5 10
    240605231500_8sns GTGTCTGTGTGCATCTATACACATGAGTTCAG(+CAGTGT)GTACCCTGTGGGAACCAGGAAAGGTCACTGA NGG 10 8 17 8 24 0 100 5 10
    240605231459_rppv AAAGCCATCTGGTTGCCTGTGAACCATTTTAG(+CAGTGT)GTACCATTATGGACTAGGCTATTTTACCTGG NGG 10 8 17 8 24 0 100 5 10
    240605231457_li1c ATTAACCAGGTAAAATAGCCTAGTCCATAATG(+CAGTGT)GTACCTAAAATGGTTCACAGGCAACCAGATG NGG 10 8 17 8 24 0 100 5 10
    240605231456_cawm TAATGCCATAAATTTTAAATTGGAAGCACATG(+CAGTGT)GATCCCATTTTTCTTTCTACTCCCACATTTT NGG 10 8 17 8 24 0 100 5 10
    240605231455_d1vy GTGAAAAAATGTGGGAGTAGAAAGAAAAATGG(+CAGTGT)GATCCATGTGCTTCCAATTTAAAATTTATGG NGG 10 8 17 8 24 0 100 5 10
    240605231452_y866 GGCGGGAAACATGGAGGCCCGGCGGTAAGGGG(+CAGTGT)GATCCCGGGCGGGCCAATCTACAAATACGCG NGG 10 8 17 8 24 0 100 5 10
    240605231449_nlf7 GGGGACGCGTATTTGTAGATTGGCCCGCCCGG(+CAGTGT)GATCCCCCTTACCGCCGGGCCTCCATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605231448_dr5l CCAACAGGGAAAAGTCACCTGACTCCATTTAG(+CAGTGT)GTACCCCAAAATTTCCCAACCATGTTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240605231446_78y5 GGTGTATAGAAACATGGTTGGGAAATTTTGGG(+CAGTGT)GTACCTAAATGGAGTCAGGTGACTTTTCCCT NGG 10 8 17 8 24 0 100 5 10