Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231415_x73x CACTTAGGTCAGTGTATCAGGCTTGGTGGCAG(+CAGTGT)GTACCTGTTCATGCTGAGGCATCGTGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605231413_x1pz GTGAGGCTGGCACGATGCCTCAGCATGAACAG(+CAGTGT)GTACCTGCCACCAAGCCTGATACACTGACCT NGG 10 8 17 8 24 0 100 5 10
    240605231412_nbse ATGGCCTTGACTCCTTTGTGGCTGAGCTGTTG(+CAGTGT)GTACCAGTGCAGTTCACGGGTGTTAGGACTG NGG 10 8 17 8 24 0 100 5 10
    240605231411_8ol9 TCCTCCAGTCCTAACACCCGTGAACTGCACTG(+CAGTGT)GTACCAACAGCTCAGCCACAAAGGAGTCAAG NGG 10 8 17 8 24 0 100 5 10
    240605231409_wvz6 TCAAGGAAGTAGATAATCAACAGTGGGAACTG(+CAGTGT)GTACCCTCTGTCCCCAGGCCTGATTTAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605231408_xw1h GGGACCTTCTAAATCAGGCCTGGGGACAGAGG(+CAGTGT)GTACCAGTTCCCACTGTTGATTATCTACTTC NGG 10 8 17 8 24 0 100 5 10
    240605231407_sgn5 TGTTTGAAATTTTAGATTTCGGGAAAGAGATG(+CAGTGT)GATCCCTGGGCTCAAAAGACAGAAATCAAGC NGG 10 8 17 8 24 0 100 5 10
    240605231404_vys8 CTTTAGCTTGATTTCTGTCTTTTGAGCCCAGG(+CAGTGT)GATCCATCTCTTTCCCGAAATCTAAAATTTC NGG 10 8 17 8 24 0 100 5 10
    240605231401_fqqj CCTTAAGGAGAGGACAGACTACTCCGACCCAG(+CAGTGT)GATCCCAAAGCTGTCCCTGCTTATTCCTTTT NGG 10 8 17 8 24 0 100 5 10
    240605231359_oshv TAAGGAAAAGGAATAAGCAGGGACAGCTTTGG(+CAGTGT)GATCCTGGGTCGGAGTAGTCTGTCCTCTCCT NGG 10 8 17 8 24 0 100 5 10