Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231357_omgf TTCAAGCCTAAAGACCTGAGTTTAGTCCTTAG(+CAGTGT)GATCCACAAGCTGGAAAAAGAAAACCAACTC NGG 10 8 17 8 24 0 100 5 10
    240605231356_uszb TTGTGGAGTTGGTTTTCTTTTTCCAGCTTGTG(+CAGTGT)GATCCTAAGGACTAAACTCAGGTCTTTAGGC NGG 10 8 17 8 24 0 100 5 10
    240605231355_bzae CTGGAAGGTCTCATGCAATCCAAGCTGACTTG(+CAGTGT)GATCCTCTTGTCTCAGCCTCTTGAGTAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605231354_ilyw TGTCCTAGCTACTCAAGAGGCTGAGACAAGAG(+CAGTGT)GATCCAAGTCAGCTTGGATTGCATGAGACCT NGG 10 8 17 8 24 0 100 5 10
    240605231352_zizy CTGCTAATCCCCTTCATCTTCCCAGTTGGTGG(+CAGTGT)GATCCAGTCAATTGCATTAGAATTGTTACTG NGG 10 8 17 8 24 0 100 5 10
    240605231350_mzvu TACTCCAGTAACAATTCTAATGCAATTGACTG(+CAGTGT)GATCCCACCAACTGGGAAGATGAAGGGGATT NGG 10 8 17 8 24 0 100 5 10
    240605231348_0a9j TCCCTCCCCACCGACATGACTCCCAAAGTGGG(+CAGTGT)GTACCAGACAGCATGGGGGTTAAGATTAGTT NGG 10 8 17 8 24 0 100 5 10
    240605231347_y1bu TAGGAAACTAATCTTAACCCCCATGCTGTCTG(+CAGTGT)GTACCCCACTTTGGGAGTCATGTCGGTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605231344_i83g GCTGGTTCCAGCTGCTGTTCCATGGGCCCTTG(+CAGTGT)GTACCAAGGACTCGTATGTGTCCCCATTTGA NGG 10 8 17 8 24 0 100 5 10
    240605231342_bsbr GCCCTTCAAATGGGGACACATACGAGTCCTTG(+CAGTGT)GTACCAAGGGCCCATGGAACAGCAGCTGGAA NGG 10 8 17 8 24 0 100 5 10