Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231252_l65m CAGTGTTCAAACCATACTAACACGACTTGATG(+CAGTGT)GTACCCAAAAATATCAACCAACAAAATTTCA NGG 10 8 17 8 24 0 100 5 10
    240605231250_mx6u GAAAATGAAATTTTGTTGGTTGATATTTTTGG(+CAGTGT)GTACCATCAAGTCGTGTTAGTATGGTTTGAA NGG 10 8 17 8 24 0 100 5 10
    240605231249_br1z ATCTAGAGGAGGACCTCTAATTTACATACATG(+CAGTGT)GATCCATCAACATTCATACATTTATTTACTT NGG 10 8 17 8 24 0 100 5 10
    240605231248_v1wc GATGGAAGTAAATAAATGTATGAATGTTGATG(+CAGTGT)GATCCATGTATGTAAATTAGAGGTCCTCCTC NGG 10 8 17 8 24 0 100 5 10
    240605231246_rsyw AGATGGTCACCAACTCTGTAATCTGTAGAGTG(+CAGTGT)GATCCACTTGCTCGAGTCACTGGGTCACACG NGG 10 8 17 8 24 0 100 5 10
    240605231245_8vzs CTATTCGTGTGACCCAGTGACTCGAGCAAGTG(+CAGTGT)GATCCACTCTACAGATTACAGAGTTGGTGAC NGG 10 8 17 8 24 0 100 5 10
    240605231244_vcm8 TCTGAAGTAGTAAAGTCCTTTTTCCAGGTTAG(+CAGTGT)GTACCAGTTCAGGGTAAGTAACTGGAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240605231242_czqw GTAGGGTCTCTCCAGTTACTTACCCTGAACTG(+CAGTGT)GTACCTAACCTGGAAAAAGGACTTTACTACT NGG 10 8 17 8 24 0 100 5 10
    240605231240_hkvp GCTCACAAACATCTATAAATGTAGCTCCATAG(+CAGTGT)GATCCAATGCCCCCATCTGCATGCAGACAAA NGG 10 8 17 8 24 0 100 5 10
    240605231239_dh2r GGCATTTTGTCTGCATGCAGATGGGGGCATTG(+CAGTGT)GATCCTATGGAGCTACATTTATAGATGTTTG NGG 10 8 17 8 24 0 100 5 10