Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231207_ocyb GGAAATGCAAGCTCAGTCAGCATCTTAAAGAG(+CAGTGT)GATCCATGTCCAAATGATGCCTGTCAGCAAG NGG 10 8 17 8 24 0 100 5 10
    240605231206_8rhl GCTACCTTGCTGACAGGCATCATTTGGACATG(+CAGTGT)GATCCTCTTTAAGATGCTGACTGAGCTTGCA NGG 10 8 17 8 24 0 100 5 10
    240605231204_qm95 AAAATGTGGAGAAGTGACAGGAATCAGAAATG(+CAGTGT)GATCCACATGAGCCGTCTGATTCTTTGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605231203_t50b ATTCAGAGGCAAAGAATCAGACGGCTCATGTG(+CAGTGT)GATCCATTTCTGATTCCTGTCACTTCTCCAC NGG 10 8 17 8 24 0 100 5 10
    240605231201_wthk CCAGTGCTCTGAACAATATGTCAAGTGCTTGG(+CAGTGT)GATCCGCTTTTAAGATGAATTTCTTGAGCAT NGG 10 8 17 8 24 0 100 5 10
    240605231200_orcq TTAATATGCTCAAGAAATTCATCTTAAAAGCG(+CAGTGT)GATCCCAAGCACTTGACATATTGTTCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605231158_5f8z AGTTTGACCCCAACACAAAGGGGAAAGACAGG(+CAGTGT)GTACCTTTCTTCATGAGTTGTCTCCCTACCC NGG 10 8 17 8 24 0 100 5 10
    240605231157_ivh6 GGGGGGGGTAGGGAGACAACTCATGAAGAAAG(+CAGTGT)GTACCCTGTCTTTCCCCTTTGTGTTGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605231155_c6n0 TGGAGAGGCCAGGACCCCACCTGCTGCATGGG(+CAGTGT)GATCCACTAGCCATGGAAGGATACAATAGGA NGG 10 8 17 8 24 0 100 5 10
    240605231154_5ycp AGGCTTCCTATTGTATCCTTCCATGGCTAGTG(+CAGTGT)GATCCCCATGCAGCAGGTGGGGTCCTGGCCT NGG 10 8 17 8 24 0 100 5 10