Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231107_e8dj GCTGGCCCATTTCATTTTGTCAGGGAGCTTAG(+CAGTGT)GTACCTGTGTCCTTGTTTTCCTAAAGCTGAT NGG 10 8 17 8 24 0 100 5 10
    240605231106_9cq4 GTGGGATCAGCTTTAGGAAAACAAGGACACAG(+CAGTGT)GTACCTAAGCTCCCTGACAAAATGAAATGGG NGG 10 8 17 8 24 0 100 5 10
    240605231104_wx9f AGATGAGAGACATCAAGGCATAGTAATTTGAG(+CAGTGT)GATCCCATAAAGCATTTCTGAGGTGGCCATG NGG 10 8 17 8 24 0 100 5 10
    240605231102_z1qc CCTGGCATGGCCACCTCAGAAATGCTTTATGG(+CAGTGT)GATCCTCAAATTACTATGCCTTGATGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605231101_3vp9 AAAAGGCATGGGGCAGGGTACACTGGGGTTTG(+CAGTGT)GATCCACTTTCCTACCTCCTTCTGCTTTGCT NGG 10 8 17 8 24 0 100 5 10
    240605231059_8ida ATGTGAGCAAAGCAGAAGGAGGTAGGAAAGTG(+CAGTGT)GATCCAAACCCCAGTGTACCCTGCCCCATGC NGG 10 8 17 8 24 0 100 5 10
    240605231057_868s TTGTTGTATTTGAACATTGTAAAGTGTGGGTG(+CAGTGT)GTACCTTAAACTGAATATGTGAAGAATCCAG NGG 10 8 17 8 24 0 100 5 10
    240605231056_mo3n AGTTTCTGGATTCTTCACATATTCAGTTTAAG(+CAGTGT)GTACCACCCACACTTTACAATGTTCAAATAC NGG 10 8 17 8 24 0 100 5 10
    240605231054_h5qd TGTGGGGTTTTAATTGGTCAGTTTTAATTTGG(+CAGTGT)GATCCCAAAGTTTTAACCTCCATTCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605231053_n196 AAGGACTTCCTGAATGGAGGTTAAAACTTTGG(+CAGTGT)GATCCCAAATTAAAACTGACCAATTAAAACC NGG 10 8 17 8 24 0 100 5 10