Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231122_28nr TTCAAAAATAGTATAGACAAGGTCAGATGCAG(+CAGTGT)GATCCATGTATAGAAAATAAGAAATCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605231120_xii1 ATGCCTGGCTGATTTCTTATTTTCTATACATG(+CAGTGT)GATCCTGCATCTGACCTTGTCTATACTATTT NGG 10 8 17 8 24 0 100 5 10
    240605231119_n6lt TGCTGGAGAAGTTGCTGAGAGTTCTATATCTG(+CAGTGT)GATCCACAGATATAGCAGGAAGAGAAAGACA NGG 10 8 17 8 24 0 100 5 10
    240605231118_jvap CCCAGTGTCTTTCTCTTCCTGCTATATCTGTG(+CAGTGT)GATCCAGATATAGAACTCTCAGCAACTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605231116_thub CTAGTCCTCCCAATGGTAGATAGATTGTCCAG(+CAGTGT)GTACCCAGGCAGGAAACCAAAAGTCCAGCAA NGG 10 8 17 8 24 0 100 5 10
    240605231114_8vqb AGGGATTGCTGGACTTTTGGTTTCCTGCCTGG(+CAGTGT)GTACCTGGACAATCTATCTACCATTGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605231113_ji6v GGAGGAGAGCCTGCAGTTCACCGGCAAGAAAG(+CAGTGT)GTACCAGTTGGAGAGAAGAAAAGCTGTACCC NGG 10 8 17 8 24 0 100 5 10
    240605231111_hyae GGTCTGGGTACAGCTTTTCTTCTCTCCAACTG(+CAGTGT)GTACCTTTCTTGCCGGTGAACTGCAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605231110_q3ga TCCAAAGGTCTGCCCACCTCCTCCAGGGCAAG(+CAGTGT)GATCCAATGTCACATCTAAGCATCTGTCCTA NGG 10 8 17 8 24 0 100 5 10
    240605231109_52wv ACCTTTAGGACAGATGCTTAGATGTGACATTG(+CAGTGT)GATCCTTGCCCTGGAGGAGGTGGGCAGACCT NGG 10 8 17 8 24 0 100 5 10