Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231017_yno7 ATGCAGAAAAGAGGCCATCCCAACATTTCCAG(+CAGTGT)GTACCTTGTTCTCCTAACACAGGCAGCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605231016_4hp0 TGAGGGTGGGCTGCCTGTGTTAGGAGAACAAG(+CAGTGT)GTACCTGGAAATGTTGGGATGGCCTCTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605231014_ixvj CGTTTGCATTGGACATAGAAGTACACCTAGAG(+CAGTGT)GATCCAAGGAAATCCACCCCCACATTACCCA NGG 10 8 17 8 24 0 100 5 10
    240605231012_4e4s TGCCTTGGGTAATGTGGGGGTGGATTTCCTTG(+CAGTGT)GATCCTCTAGGTGTACTTCTATGTCCAATGC NGG 10 8 17 8 24 0 100 5 10
    240605231011_n6ey AGAGCTGTGTAGATGATCCTGAAGACAGGCTG(+CAGTGT)GATCCTGCGGCCTCCTCTCCTGGGTACTGTC NGG 10 8 17 8 24 0 100 5 10
    240605231009_j874 AGGGAGACAGTACCCAGGAGAGGAGGCCGCAG(+CAGTGT)GATCCAGCCTGTCTTCAGGATCATCTACACA NGG 10 8 17 8 24 0 100 5 10
    240605231007_7dh9 TTGTCTTCCCTGGCTCACAGGGCTCCTGTGAG(+CAGTGT)GATCCAACGATGTGATGGGTCAGGGAGCTCG NGG 10 8 17 8 24 0 100 5 10
    240605231006_2hll GTCAACGAGCTCCCTGACCCATCACATCGTTG(+CAGTGT)GATCCTCACAGGAGCCCTGTGAGCCAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605231005_8x1o TCAAGACATGAGTATGAGCACAGTTTCAAATG(+CAGTGT)GTACCAAAATTAATCCATTTCACAGTAATCA NGG 10 8 17 8 24 0 100 5 10
    240605231004_nvf1 AGAAATGATTACTGTGAAATGGATTAATTTTG(+CAGTGT)GTACCATTTGAAACTGTGCTCATACTCATGT NGG 10 8 17 8 24 0 100 5 10