Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606110824_m00f AGCACTAGCAGAGGACCTGGGTTCAATTCCCG(+AAAAAA)GTACCCACACAGTGGCTTACAACTATCCGTA NGG 10 8 17 8 24 0 100 5 10
    240606110823_db11 TAATGTCCTACTCCTGGCTTGCAATACATGTG(+AAAAAA)GATCCTCTTCAGTTATGAATGGAAAATGCCA NGG 10 8 17 8 24 0 100 5 10
    240606110822_fwzb TTTCTTGGCATTTTCCATTCATAACTGAAGAG(+AAAAAA)GATCCACATGTATTGCAAGCCAGGAGTAGGA NGG 10 8 17 8 24 0 100 5 10
    240606110821_8674 ATAACATTAACCACATTCTGTTATATAGACTG(+AAAAAA)GATCCTTAAAATACACTAATTTATAAATACT NGG 10 8 17 8 24 0 100 5 10
    240606110820_yool TTTTCAGTATTTATAAATTAGTGTATTTTAAG(+AAAAAA)GATCCAGTCTATATAACAGAATGTGGTTAAT NGG 10 8 17 8 24 0 100 5 10
    240606110818_w91s GTTGGTGTTTACATTGCTCTGCTCTGTGAAAG(+AAAAAA)GATCCTGCAGCAGAGGCTTCCTACCTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606110817_5oyy AACATTTTTCAGGTAGGAAGCCTCTGCTGCAG(+AAAAAA)GATCCTTTCACAGAGCAGAGCAATGTAAACA NGG 10 8 17 8 24 0 100 5 10
    240606110815_k8rm GGCTGATGCCTGTCAAGATGACAACAGGAGGG(+AAAAAA)GATCCACCAAGGTAGAAAACATCCCTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606110814_n5lt TCTTGTCAGCAGGGATGTTTTCTACCTTGGTG(+AAAAAA)GATCCCCTCCTGTTGTCATCTTGACAGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606110812_k85g AGGTGAACGTTCCACTGTAAAAGGCACTACTG(+AAAAAA)GATCCGAGAGGGTTTATTTTAAGCATGTGCA NGG 10 8 17 8 24 0 100 5 10