Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230930_d7ah GTCAATTTAATGCTTTAAAAGTAACTTATCAG(+CAGTGT)GATCCACTTCAATCTCCACATGATAATAAAG NGG 10 8 17 8 24 0 100 5 10
    240605230929_mea8 TAATCCTTTATTATCATGTGGAGATTGAAGTG(+CAGTGT)GATCCTGATAAGTTACTTTTAAAGCATTAAA NGG 10 8 17 8 24 0 100 5 10
    240605230927_yfml TTGTGCTTTGTAAAGCAAACTCGAGAAAACTG(+CAGTGT)GTACCAGTGTCTGTACCTCAGCTGGGTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605230926_ioas AGATGAAGGACCCAGCTGAGGTACAGACACTG(+CAGTGT)GTACCAGTTTTCTCGAGTTTGCTTTACAAAG NGG 10 8 17 8 24 0 100 5 10
    240605230925_ftb5 ATTCAAAGTTAGAGTGAATCCATGCCAAAAGG(+CAGTGT)GATCCCTCACACTGGATAAGAGAAACCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605230923_pswd CTGATCACTGGTTTCTCTTATCCAGTGTGAGG(+CAGTGT)GATCCCTTTTGGCATGGATTCACTCTAACTT NGG 10 8 17 8 24 0 100 5 10
    240605230922_lyzc AAAGTATAAGCCTGTCAAAGCACGGACTTTAG(+CAGTGT)GATCCCCACCAAATATATATACAGATGTACA NGG 10 8 17 8 24 0 100 5 10
    240605230920_pbq7 ATATGTGTACATCTGTATATATATTTGGTGGG(+CAGTGT)GATCCTAAAGTCCGTGCTTTGACAGGCTTAT NGG 10 8 17 8 24 0 100 5 10
    240605230918_o4bj ACTGTAAAGAAAGCTTCTAGGTCTTCAAAAGG(+CAGTGT)GATCCAGAAAAGGGCAAGCCTGAAATGAGGA NGG 10 8 17 8 24 0 100 5 10
    240605230917_3j8g CTAGCTCCTCATTTCAGGCTTGCCCTTTTCTG(+CAGTGT)GATCCCTTTTGAAGACCTAGAAGCTTTCTTT NGG 10 8 17 8 24 0 100 5 10