Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231034_cipz TCCAGAGGTTTTTACTGAGAGGACTGAGGGGG(+CAGTGT)GTACCCCAGCTGGAGGGCTGAAGGCCCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605231032_njxa GCCTTTCCTGGGCCTTCAGCCCTCCAGCTGGG(+CAGTGT)GTACCCCCCTCAGTCCTCTCAGTAAAAACCT NGG 10 8 17 8 24 0 100 5 10
    240605231030_n4h9 CCTTTTAGCTTGGAGGTGGGAGCCATGCCCAG(+CAGTGT)GATCCCTTTCCCGGGCTTTGTCCATGGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605231028_yae8 AGGGATTTCCCATGGACAAAGCCCGGGAAAGG(+CAGTGT)GATCCTGGGCATGGCTCCCACCTCCAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605231027_iu0k TCTTTCCTCCAGCCCTTTTGGTCTCTTTCCTG(+CAGTGT)GATCCTTGTCCTCCAGACTGGCCTCTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605231025_mw6p ACTTTCCAGCAGAGGCCAGTCTGGAGGACAAG(+CAGTGT)GATCCAGGAAAGAGACCAAAAGGGCTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605231024_e3fu GCCTTCCTCAGCCCTGACCTACCTGGCCATAG(+CAGTGT)GATCCTACAGCCTTCTCTGTACTCCACTATC NGG 10 8 17 8 24 0 100 5 10
    240605231022_b63r CAACTGATAGTGGAGTACAGAGAAGGCTGTAG(+CAGTGT)GATCCTATGGCCAGGTAGGTCAGGGCTGAGG NGG 10 8 17 8 24 0 100 5 10
    240605231021_w59p AAACTCAGGTCCTGATATTTGTGAAACAGGTG(+CAGTGT)GTACCACTGAGCCAGGTCCATATTCCTCATT NGG 10 8 17 8 24 0 100 5 10
    240605231019_arm3 GGGTCAATGAGGAATATGGACCTGGCTCAGTG(+CAGTGT)GTACCACCTGTTTCACAAATATCAGGACCTG NGG 10 8 17 8 24 0 100 5 10