Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606110900_qbq3 GATGAAGAAGACCAGCCTTTATTAGCTGCTTG(+AAAAAA)GTACCTTCCAAGAGGTTTGGACCTCCTGTTA NGG 10 8 17 8 24 0 100 5 10
    240606110859_9ufs AGTCTCCCTAGCGCCACTGTGCTGAGAACAGG(+AAAAAA)GATCCTCTGGATGAGGAAGCCCTGGCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240606110857_7w7b CTGAGCTGGGGCCAGGGCTTCCTCATCCAGAG(+AAAAAA)GATCCCTGTTCTCAGCACAGTGGCGCTAGGG NGG 10 8 17 8 24 0 100 5 10
    240606110855_fdqh CATCTAACAGCAGCTTGAACCTTGGATCTTAG(+AAAAAA)GATCCGCTTGACATAGTCTGCTTTCTCGGAG NGG 10 8 17 8 24 0 100 5 10
    240606110854_1xmo AAGTTCTCCGAGAAAGCAGACTATGTCAAGCG(+AAAAAA)GATCCTAAGATCCAAGGTTCAAGCTGCTGTT NGG 10 8 17 8 24 0 100 5 10
    240606110852_uabr AGTGTCTGGGTTTGGTGACTGATTATGGGATG(+AAAAAA)GATCCGGGTGGGGTATGCTCTGGATAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240606110847_uh6f AAGGATGAACTATCCAGAGCATACCCCACCCG(+AAAAAA)GATCCATCCCATAATCAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606110844_7oux TCTTTCTCTAGCTCTTCCATTGGGGGACCTGG(+AAAAAA)GATCCATCCAATAGCTGACTGTGAGCATCCA NGG 10 8 17 8 24 0 100 5 10
    240606110842_jxpj AGAAGTGGATGCTCACAGTCAGCTATTGGATG(+AAAAAA)GATCCCAGGTCCCCCAATGGAAGAGCTAGAG NGG 10 8 17 8 24 0 100 5 10
    240606110841_par2 CTAATGGTGCGTTTGTTTCTGCTTTTGACTTG(+AAAAAA)GTACCTGTGCACTCTAAGCAGGTAGGCCCTG NGG 10 8 17 8 24 0 100 5 10