Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230945_pw5e TGCAAGAAGAACACTGCATACTGGGCATGCAG(+CAGTGT)GTACCACCACCTGTCAGCAGTTTCTCCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605230943_kuxn GCAGAGCCTGGAGAAACTGCTGACAGGTGGTG(+CAGTGT)GTACCTGCATGCCCAGTATGCAGTGTTCTTC NGG 10 8 17 8 24 0 100 5 10
    240605230942_nbag ACCCAGCTAGTGAGCTGTGCTCTGCAGGAGTG(+CAGTGT)GATCCGAGTCTCCTAGATTGAGATTTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605230941_4ees GTGCACCTCCAAATCTCAATCTAGGAGACTCG(+CAGTGT)GATCCACTCCTGCAGAGCACAGCTCACTAGC NGG 10 8 17 8 24 0 100 5 10
    240605230939_gm59 AACTGTTCCCAAACATCAAGAATCTTTCCAAG(+CAGTGT)GATCCTAAGGTGAAGCACTACCTACGATTTT NGG 10 8 17 8 24 0 100 5 10
    240605230937_mkgm AAGTGAAAATCGTAGGTAGTGCTTCACCTTAG(+CAGTGT)GATCCTTGGAAAGATTCTTGATGTTTGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605230936_9o3n GTAGAGTTTTTATAAGGCCAGTGAGTTAACTG(+CAGTGT)GTACCCTGTACTGCCAAATCTAATTTTTAGG NGG 10 8 17 8 24 0 100 5 10
    240605230934_8q7s CTAACCCTAAAAATTAGATTTGGCAGTACAGG(+CAGTGT)GTACCAGTTAACTCACTGGCCTTATAAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605230932_y3gu CTAACCCTAAAAATTAGATTTGGCAGTACAGG(+CAGTGT)GTACCAGTTAACTCACTGGCCTTATAAAAAC NGG 10 8 17 8 24 0 100 5 10
    240605230931_caxf GTAGAGTTTTTATAAGGCCAGTGAGTTAACTG(+CAGTGT)GTACCCTGTACTGCCAAATCTAATTTTTAGG NGG 10 8 17 8 24 0 100 5 10