Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230856_lani GGATTCTCAGGAGCTTCTCTCCCTACCCAATG(+CAGTGT)GATCCACCCCCATCACACACATTTACATACA NGG 10 8 17 8 24 0 100 5 10
    240605230854_8jya GGGCTTGTATGTAAATGTGTGTGATGGGGGTG(+CAGTGT)GATCCATTGGGTAGGGAGAGAAGCTCCTGAG NGG 10 8 17 8 24 0 100 5 10
    240605230853_u4ym AACTGAAGTGATACAGTTCAAATGCCAGCGAG(+CAGTGT)GATCCATGCTTGAAGACAAGTATTAGGATAA NGG 10 8 17 8 24 0 100 5 10
    240605230852_bo2y ATCATTTATCCTAATACTTGTCTTCAAGCATG(+CAGTGT)GATCCTCGCTGGCATTTGAACTGTATCACTT NGG 10 8 17 8 24 0 100 5 10
    240605230850_izde CTGCCCTTAAATGATCTGGACTCACCTGGGAG(+CAGTGT)GTACCTGGGCTGCCCAAGGCCCATGGACATT NGG 10 8 17 8 24 0 100 5 10
    240605230847_c5ps GAATCAATGTCCATGGGCCTTGGGCAGCCCAG(+CAGTGT)GTACCTCCCAGGTGAGTCCAGATCATTTAAG NGG 10 8 17 8 24 0 100 5 10
    240605230846_nbih CTGCCCTTAACTGATCCGGACTCACCTGGGAG(+CAGTGT)GTACCTGGGCTGCCCACGGCCCATGGACATT NGG 10 8 17 8 24 0 100 5 10
    240605230843_96ju GAATCAATGTCCATGGGCCGTGGGCAGCCCAG(+CAGTGT)GTACCTCCCAGGTGAGTCCGGATCAGTTAAG NGG 10 8 17 8 24 0 100 5 10
    240605230841_syqd GAATCAATGTCCATGGGCCGTGGGCAGCCCAG(+CAGTGT)GTACCTCCCAGGTGAGTCCGGGTCAGTTAAG NGG 10 8 17 8 24 0 100 5 10
    240605230839_t9jg CTGCCCTTAACTGACCCGGACTCACCTGGGAG(+CAGTGT)GTACCTGGGCTGCCCACGGCCCATGGACATT NGG 10 8 17 8 24 0 100 5 10