Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230749_90qq GAAACAACCCAAGCTCCTCCTCCGTCCCTCTG(+CAGTGT)GTACCAGATCTAACCAGGCATCCAAAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605230747_ec55 CTCCTAGGCCTTTGGATGCCTGGTTAGATCTG(+CAGTGT)GTACCAGAGGGACGGAGGAGGAGCTTGGGTT NGG 10 8 17 8 24 0 100 5 10
    240605230745_0bjm ATTGCCAGCCAATCCTTTTAGGAATAGGGCAG(+CAGTGT)GTACCTTCCACTTGTATTTATTTCTGTAGCT NGG 10 8 17 8 24 0 100 5 10
    240605230744_5w8c AGAGAAGCTACAGAAATAAATACAAGTGGAAG(+CAGTGT)GTACCTGCCCTATTCCTAAAAGGATTGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605230742_0pux AGGAAGGACTGCCCTTGCTATTGGAGTCAAGG(+CAGTGT)GTACCCCAGTAATAGTTGTGATAATCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605230740_b4jw AGGATCAGGAGATTATCACAACTATTACTGGG(+CAGTGT)GTACCCTTGACTCCAATAGCAAGGGCAGTCC NGG 10 8 17 8 24 0 100 5 10
    240605230739_vt20 GTGGGAGCTGGCTGGCGCCTCTTCTACTGCTG(+CAGTGT)GTACCCCTACCAGGGACCGGGCCTTCTCCGT NGG 10 8 17 8 24 0 100 5 10
    240605230737_e5zg CAAAGACGGAGAAGGCCCGGTCCCTGGTAGGG(+CAGTGT)GTACCAGCAGTAGAAGAGGCGCCAGCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605230736_vkr7 CCTCACTTGGATGATTTCATTCCTCTCACAAG(+CAGTGT)GATCCCTTTGGCCCATCCTCCTGGCCACTCT NGG 10 8 17 8 24 0 100 5 10
    240605230734_q8kb TCCAAAGAGTGGCCAGGAGGATGGGCCAAAGG(+CAGTGT)GATCCTTGTGAGAGGAATGAAATCATCCAAG NGG 10 8 17 8 24 0 100 5 10