Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230641_wids AAATGTCCCAAGTCCCACAGTATTGGAGCTGG(+CAGTGT)GTACCAGGGCCAGCCTGGTTCTTTGGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605230639_kz95 GAGAAAGAGACCAAAGAACCAGGCTGGCCCTG(+CAGTGT)GTACCCAGCTCCAATACTGTGGGACTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605230637_yhd4 CACTGATACTGAGAAGGTGTTGGAAAACACAG(+CAGTGT)GATCCATAATCTGTTGGAATAGCGGGAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605230636_684k ACACAACTTTCCCGCTATTCCAACAGATTATG(+CAGTGT)GATCCTGTGTTTTCCAACACCTTCTCAGTAT NGG 10 8 17 8 24 0 100 5 10
    240605230634_arju TCTGGGGGTGATTCCGTGTTAATCCTTGGAGG(+CAGTGT)GTACCTTTCTGTTCTGATTTTTTTGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605230633_zr9f AAACAAACAAACAAAAAAATCAGAACAGAAAG(+CAGTGT)GTACCCTCCAAGGATTAACACGGAATCACCC NGG 10 8 17 8 24 0 100 5 10
    240605230631_s365 CTTACTTTCTCCCTTTAACATACATAGGCCAG(+CAGTGT)GATCCAAGCCTAATGAATAGTGTCACCTATG NGG 10 8 17 8 24 0 100 5 10
    240605230630_ki3g CCCACCATAGGTGACACTATTCATTAGGCTTG(+CAGTGT)GATCCTGGCCTATGTATGTTAAAGGGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605230628_pvsf AGTGATCTGTGTGTATGAGAAAGAGAAGGAAG(+CAGTGT)GTACCTCTCACTGTTTTAAATTTGCAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240605230627_ub2q CAACCATTTCTGCAAATTTAAAACAGTGAGAG(+CAGTGT)GTACCTTCCTTCTCTTTCTCATACACACAGA NGG 10 8 17 8 24 0 100 5 10