Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231855_2x31 GGGCCACATTTTAGCCCTGATGATCCTCCTGG(+CAGTGT)GTACCTTATGCAGGGTCCTCATGAAGGGTCC NGG 10 8 17 8 24 0 100 5 10
    240605231854_lwau AAGCAAAAGTATGTCGCCTTTTTCTGAACATG(+CAGTGT)GTACCATAGGAATGCTGGTGGCTCTGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240605231852_1bw8 CACCTCTACACAGAGCCACCAGCATTCCTATG(+CAGTGT)GTACCATGTTCAGAAAAAGGCGACATACTTT NGG 10 8 17 8 24 0 100 5 10
    240605231851_79zn TCAAAGCAGGAGAAACTGAAAAGTCACAGCTG(+CAGTGT)GTACCAAAGAGCAGGCCAGACTTCACAGCGG NGG 10 8 17 8 24 0 100 5 10
    240605231850_1yrx GAGACCCGCTGTGAAGTCTGGCCTGCTCTTTG(+CAGTGT)GTACCAGCTGTGACTTTTCAGTTTCTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605231847_jo3x TTGGACCTATAGACTGTATTTACGATCCCATG(+CAGTGT)GATCCTATCTTACACATAGTAGATATGAAAT NGG 10 8 17 8 24 0 100 5 10
    240605231845_p6sh TAGATATTTCATATCTACTATGTGTAAGATAG(+CAGTGT)GATCCATGGGATCGTAAATACAGTCTATAGG NGG 10 8 17 8 24 0 100 5 10
    240605231844_zj0b TGCACCACTGTGTCTTTCCCAATGTTTTTATG(+CAGTGT)GTACCTCGGCTTTTGAGGCAGAGTGTGCAAA NGG 10 8 17 8 24 0 100 5 10
    240605231842_8ll9 CTCGGTTTGCACACTCTGCCTCAAAAGCCGAG(+CAGTGT)GTACCATAAAAACATTGGGAAAGACACAGTG NGG 10 8 17 8 24 0 100 5 10
    240605231840_5m8l CTACTATTCAATGACAAATCCCCCAGAGCTGG(+CAGTGT)GATCCCAGGGGTACGCTACATCCAGCTGGTG NGG 10 8 17 8 24 0 100 5 10