Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230503_9are AAACACGGCTTTCCTAAGTAACTGTACCAATG(+CAGTGT)GATCCTAAAGTCCCAAATGTAGGCCCTTGAA NGG 10 8 17 8 24 0 100 5 10
    240605230501_9mkt TTTGTTTCAAGGGCCTACATTTGGGACTTTAG(+CAGTGT)GATCCATTGGTACAGTTACTTAGGAAAGCCG NGG 10 8 17 8 24 0 100 5 10
    240605230500_4kuh GCTTTTTTCCCCTTCTAAAGTTCAAGTAAAGG(+CAGTGT)GTACCTTTAAGAACAATCTGAATAGGGACTG NGG 10 8 17 8 24 0 100 5 10
    240605230458_pore GCCTGCAGTCCCTATTCAGATTGTTCTTAAAG(+CAGTGT)GTACCCTTTACTTGAACTTTAGAAGGGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605230457_8jgs ACCACATGGTGGCTCAGTACCATCTGTAATGG(+CAGTGT)GATCCAATTCCCTCTTCTAGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605230456_j6j4 GCTGTCTTCAGACACACTAGAAGAGGGAATTG(+CAGTGT)GATCCCATTACAGATGGTACTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605230454_vb26 TGTATGTCAGTGATGGGGCTTGCCCGGGGAAG(+CAGTGT)GATCCAGAGATGAAGTAGAAGGAAAAGACTC NGG 10 8 17 8 24 0 100 5 10
    240605230453_y14w GCAGGGAGTCTTTTCCTTCTACTTCATCTCTG(+CAGTGT)GATCCTTCCCCGGGCAAGCCCCATCACTGAC NGG 10 8 17 8 24 0 100 5 10
    240605230451_ccm6 ACGTAGGCATGCAGTCAGTAGAAGGAGGGAGG(+CAGTGT)GTACCAACTGTGGTCAGGAGCTGTTCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605230449_sig9 CTACTTCCCAGAACAGCTCCTGACCACAGTTG(+CAGTGT)GTACCCTCCCTCCTTCTACTGACTGCATGCC NGG 10 8 17 8 24 0 100 5 10