Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230430_n63d TCCTCCAAGCTCTCAGGAGGCAGAGGCAGGTG(+CAGTGT)GATCCCTGTGAGTTTGAGGCCAGGGCTACAC NGG 10 8 17 8 24 0 100 5 10
    240605230428_ap3g TCACTGTGTAGCCCTGGCCTCAAACTCACAGG(+CAGTGT)GATCCACCTGCCTCTGCCTCCTGAGAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605230426_por2 TGTTAGCATCGGAAATTGAGGCAAAACCATTG(+CAGTGT)GATCCAAGAAGCCAGGCCTGTTTCTTTAACA NGG 10 8 17 8 24 0 100 5 10
    240605230425_abss ATAGTTGTTAAAGAAACAGGCCTGGCTTCTTG(+CAGTGT)GATCCAATGGTTTTGCCTCAATTTCCGATGC NGG 10 8 17 8 24 0 100 5 10
    240605230423_wfse TAGGAAGATATTACCAACCCTGCTCAAACAGG(+CAGTGT)GTACCAAAGGAACGGTTAAGGTCCCCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605230421_eid3 GGTTAAGAGAGGGGACCTTAACCGTTCCTTTG(+CAGTGT)GTACCCTGTTTGAGCAGGGTTGGTAATATCT NGG 10 8 17 8 24 0 100 5 10
    240605230420_zclt TGTTCCAAGACACACCCCTTCTAGAGATGCTG(+CAGTGT)GATCCAACCCAAACCTGCCCACTCCGGAACC NGG 10 8 17 8 24 0 100 5 10
    240605230418_ovp1 TGGTGGGTTCCGGAGTGGGCAGGTTTGGGTTG(+CAGTGT)GATCCAGCATCTCTAGAAGGGGTGTGTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605230416_4rjw TATTGGATGGGCACTGGGGGCCAGGGGCCTTG(+CAGTGT)GATCCCAAATAAAAGAGAAGTTCTTCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605230415_5v20 GTGGCCCACAGAAGAACTTCTCTTTTATTTGG(+CAGTGT)GATCCAAGGCCCCTGGCCCCCAGTGCCCATC NGG 10 8 17 8 24 0 100 5 10