Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230340_94sy GGATGGACCATCTAGAGACTGCCATATCTGGG(+CAGTGT)GATCCATCCCATAATCAGCCTCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605230338_n7jj GGTGTCAGCGTTTGGAGGCTGATTATGGGATG(+CAGTGT)GATCCCCAGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605230337_uufx CTAAACAAACTTGGAGGTAGTCACTCAGGATG(+CAGTGT)GATCCCAATTCACACTTCTCCATAGTTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605230335_c8qg CAATCATGGAACTATGGAGAAGTGTGAATTGG(+CAGTGT)GATCCATCCTGAGTGACTACCTCCAAGTTTG NGG 10 8 17 8 24 0 100 5 10
    240605230334_grvz GGAGGCCTAGAGAGCTCACAGTATTGAGTGAG(+CAGTGT)GTACCCCTTCCACTGGTTTCCATAGCACCCC NGG 10 8 17 8 24 0 100 5 10
    240605230332_x2z7 GAAATGGGGTGCTATGGAAACCAGTGGAAGGG(+CAGTGT)GTACCTCACTCAATACTGTGAGCTCTCTAGG NGG 10 8 17 8 24 0 100 5 10
    240605230330_juqd GTCCAGGTCTATATATAGTCAACTCTCTTAGG(+CAGTGT)GATCCCCATCTCCCTTCCTCTGGGCCTTGCT NGG 10 8 17 8 24 0 100 5 10
    240605230328_zv9t TACAGAGCAAGGCCCAGAGGAAGGGAGATGGG(+CAGTGT)GATCCCTAAGAGAGTTGACTATATATAGACC NGG 10 8 17 8 24 0 100 5 10
    240605230326_bjow AAGTTCTTGTTCTCTGAGTCAGAGCTGGGTGG(+CAGTGT)GTACCACTGTGCTGGTACTGAAAGGCGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605230325_uv31 CCTGCCCCACGCCTTTCAGTACCAGCACAGTG(+CAGTGT)GTACCCACCCAGCTCTGACTCAGAGAACAAG NGG 10 8 17 8 24 0 100 5 10