Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230323_tmi1 TAGAGACTGCCAACAGTGGGAGCTACTCCCTG(+CAGTGT)GATCCCTCTTGGGCCGAGTAGTTTTTAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605230321_s0uw ATTTGGCTTTAAAAACTACTCGGCCCAAGAGG(+CAGTGT)GATCCAGGGAGTAGCTCCCACTGTTGGCAGT NGG 10 8 17 8 24 0 100 5 10
    240605230320_d4el TGGTGGAACCTGTACACTGAAGAGTTAGTGAG(+CAGTGT)GTACCCCAAATTACAGCAGTACCCCCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605230318_3lma CTCCAGAGAAGGGGGTACTGCTGTAATTTGGG(+CAGTGT)GTACCTCACTAACTCTTCAGTGTACAGGTTC NGG 10 8 17 8 24 0 100 5 10
    240605230316_8rvv CAATATCACGACGTGGAAATAGGAAGTTTGAG(+CAGTGT)GATCCCCAGAGCAGCCAGCCTTCACGGGTTC NGG 10 8 17 8 24 0 100 5 10
    240605230314_qxhf CCCAGGAACCCGTGAAGGCTGGCTGCTCTGGG(+CAGTGT)GATCCTCAAACTTCCTATTTCCACGTCGTGA NGG 10 8 17 8 24 0 100 5 10
    240605230312_wg3j AGGCTACGTGCAGTATCTGCAATTCACGGATG(+CAGTGT)GATCCATCACTTTGGGTCTGAGGTACGGCTA NGG 10 8 17 8 24 0 100 5 10
    240605230311_b8d6 ATGTTTAGCCGTACCTCAGACCCAAAGTGATG(+CAGTGT)GATCCATCCGTGAATTGCAGATACTGCACGT NGG 10 8 17 8 24 0 100 5 10
    240605230309_hpki GTACAATGGTGGCTTTAGCAACCACAAAGCTG(+CAGTGT)GATCCAGAAGTACTCATTAGTGTTGTATTTG NGG 10 8 17 8 24 0 100 5 10
    240605230308_1bvb AGATGCAAATACAACACTAATGAGTACTTCTG(+CAGTGT)GATCCAGCTTTGTGGTTGCTAAAGCCACCAT NGG 10 8 17 8 24 0 100 5 10