Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230250_fe8x TGTGGATTTGTTATATTTTAATATGTGAAATG(+CAGTGT)GATCCAGTCATTGGAACAGATGTGTGAAGCT NGG 10 8 17 8 24 0 100 5 10
    240605230249_j9or CCCAGAGCTTCACACATCTGTTCCAATGACTG(+CAGTGT)GATCCATTTCACATATTAAAATATAACAAAT NGG 10 8 17 8 24 0 100 5 10
    240605230247_btpo TTAAGAGTGTGGTGTGTCTCCAGCTATGTCTG(+CAGTGT)GATCCTCTATGGAAAAGCCGTATTCATTAAA NGG 10 8 17 8 24 0 100 5 10
    240605230246_4n0d GGAGCTTTAATGAATACGGCTTTTCCATAGAG(+CAGTGT)GATCCAGACATAGCTGGAGACACACCACACT NGG 10 8 17 8 24 0 100 5 10
    240605230244_d53l TCAAGTTAACAATGAAAACCAAACCTCTCCAG(+CAGTGT)GATCCCTTCCAGTGCTGTTTCTTACGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240605230242_q9x5 TCTTTAACCCCGTAAGAAACAGCACTGGAAGG(+CAGTGT)GATCCTGGAGAGGTTTGGTTTTCATTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605230241_4415 AGGTTTTGCTTCCTTCTCATCTCTGCCTTGAG(+CAGTGT)GATCCTTTTTCTTGGACCGGGGTGGAAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605230240_99hs TGTCCTCTCTTCCACCCCGGTCCAAGAAAAAG(+CAGTGT)GATCCTCAAGGCAGAGATGAGAAGGAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240605230238_l91d ATTCAGTCAGATGATGGCAAGAGGCTGCTGGG(+CAGTGT)GTACCACATGACACAGCTAATGTTTAAATAA NGG 10 8 17 8 24 0 100 5 10
    240605230237_3kzc TATTATTATTTAAACATTAGCTGTGTCATGTG(+CAGTGT)GTACCCCAGCAGCCTCTTGCCATCATCTGAC NGG 10 8 17 8 24 0 100 5 10