Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230200_fa4s TGCTGGAAGCCCTGGGTTTCACCTCCAGAACG(+CAGTGT)GTACCTGTGATCACAACACTCAAGAGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240605230159_95g6 CTGCCTCTACCTCTTGAGTGTTGTGATCACAG(+CAGTGT)GTACCGTTCTGGAGGTGAAACCCAGGGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605230158_uf0t CACTATCACCGACATTCCTTCCAGCCACACTG(+CAGTGT)GATCCAGGAAACCTGTGCTGTTACAGCTAAA NGG 10 8 17 8 24 0 100 5 10
    240605230156_j649 GAACATTTAGCTGTAACAGCACAGGTTTCCTG(+CAGTGT)GATCCAGTGTGGCTGGAAGGAATGTCGGTGA NGG 10 8 17 8 24 0 100 5 10
    240605230154_nlej AGAAATACAGAAATGGAAGTCAAACCGCCGAG(+CAGTGT)GATCCTGCGGCGGTGACCTCGGGGCTCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605230152_iwgh CTTCGTCCAGAGCCCCGAGGTCACCGCCGCAG(+CAGTGT)GATCCTCGGCGGTTTGACTTCCATTTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240605230150_rla8 CAGAGTCCGCTGCCTTATCTATCAGCAGAAGG(+CAGTGT)GATCCAGGTGGTTCTTGGAACTCAGATAAGC NGG 10 8 17 8 24 0 100 5 10
    240605230148_r0qj GGGAAGCTTATCTGAGTTCCAAGAACCACCTG(+CAGTGT)GATCCCTTCTGCTGATAGATAAGGCAGCGGA NGG 10 8 17 8 24 0 100 5 10
    240605230146_ced9 GCTTGCTGTGCAGGCATGAGGGTGTACACTTG(+CAGTGT)GATCCCCAGAACCCACATAATAGCTGGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605230144_z2bc CAGCTGTGCCCAGCTATTATGTGGGTTCTGGG(+CAGTGT)GATCCAAGTGTACACCCTCATGCCTGCACAG NGG 10 8 17 8 24 0 100 5 10