Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230112_ljiy TCATGACTACAGCTCTGGGTCACCTCCTGTGG(+CAGTGT)GATCCACCAGGGGACGGGTTACATCTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605230110_iiw1 GGCTTCAGGAAGATGTAACCCGTCCCCTGGTG(+CAGTGT)GATCCCACAGGAGGTGACCCAGAGCTGTAGT NGG 10 8 17 8 24 0 100 5 10
    240605230108_jvic CAAAAGACAAAGATGCCTTTTCAAAATGGCGG(+CAGTGT)GATCCACCTGGAGGAAATCCTAGTGAAACCA NGG 10 8 17 8 24 0 100 5 10
    240605230106_bzb6 GGAAGTGGTTTCACTAGGATTTCCTCCAGGTG(+CAGTGT)GATCCCGCCATTTTGAAAAGGCATCTTTGTC NGG 10 8 17 8 24 0 100 5 10
    240605230105_psyc TGTTTTTTCCTTTGTTGACTTCACTTGTAGTG(+CAGTGT)GTACCATTTTTGGGATGTGTACTGTTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240605230104_y7gg TTCCCATAGAAACAGTACACATCCCAAAAATG(+CAGTGT)GTACCACTACAAGTGAAGTCAACAAAGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605230102_k0b6 GGCTTGTTGGTAAAACCATTGTTTGGGTTTGG(+CAGTGT)GTACCAATCGTTTGTAGCTTAGTTTTAGATC NGG 10 8 17 8 24 0 100 5 10
    240605230100_lobr ATAGTGATCTAAAACTAAGCTACAAACGATTG(+CAGTGT)GTACCCAAACCCAAACAATGGTTTTACCAAC NGG 10 8 17 8 24 0 100 5 10
    240605230059_gzm0 TGTCAACAGCCCTAAGCCGCCTCACCCGAGAG(+CAGTGT)GTACCTTCCAGAGGGGAGGCAGATGTCCTCA NGG 10 8 17 8 24 0 100 5 10
    240605230058_6n42 GACAATGAGGACATCTGCCTCCCCTCTGGAAG(+CAGTGT)GTACCTCTCGGGTGAGGCGGCTTAGGGCTGT NGG 10 8 17 8 24 0 100 5 10