Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230019_ylbq GAAGTCTGTGGAGCCAGTGAGGAAGACTCCTG(+CAGTGT)GATCCCTCCCCGTGATGGGTTAACCCCACAG NGG 10 8 17 8 24 0 100 5 10
    240605230017_hwyg CAGCTCTGTGGGGTTAACCCATCACGGGGAGG(+CAGTGT)GATCCAGGAGTCTTCCTCACTGGCTCCACAG NGG 10 8 17 8 24 0 100 5 10
    240605230016_e39h CAGAAAGTACCGCTTCCACTGAGACAGTGCTG(+CAGTGT)GATCCTGCCGAGCACTGCTGATCCCAGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605230014_18us TGCATCCCTCTGGGATCAGCAGTGCTCGGCAG(+CAGTGT)GATCCAGCACTGTCTCAGTGGAAGCGGTACT NGG 10 8 17 8 24 0 100 5 10
    240605230013_kwof ATGGCAGAGGAAGAGTCTCGCAGCCTGGAGTG(+CAGTGT)GATCCCAGCAGGGACTATAAAAGTTGTCATT NGG 10 8 17 8 24 0 100 5 10
    240605230011_u3wz GGGGAAATGACAACTTTTATAGTCCCTGCTGG(+CAGTGT)GATCCACTCCAGGCTGCGAGACTCTTCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605230009_hkb3 GTGGAACGGCCGGTGTGAGCCGCAGGTCTGTG(+CAGTGT)GATCCATCCCCGAGGGCGGGGCAGTAATTCA NGG 10 8 17 8 24 0 100 5 10
    240605230007_g1xd GGCTGTGAATTACTGCCCCGCCCTCGGGGATG(+CAGTGT)GATCCACAGACCTGCGGCTCACACCGGCCGT NGG 10 8 17 8 24 0 100 5 10
    240605230005_45ru TACATATCCCATGGGGGAAACCATACAGGGAG(+CAGTGT)GTACCCTAATCATTCTCCCATACCCTTAGTG NGG 10 8 17 8 24 0 100 5 10
    240605230004_pqfk CGTGGCACTAAGGGTATGGGAGAATGATTAGG(+CAGTGT)GTACCTCCCTGTATGGTTTCCCCCATGGGAT NGG 10 8 17 8 24 0 100 5 10