Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230037_ty6w GGGGGTCCGTGCAAGTGCTAACATATCTCCAG(+CAGTGT)GATCCTCGGTGGTGCTAACCTATCTCCATGT NGG 10 8 17 8 24 0 100 5 10
    240605230035_gslh GAGAAACATGGAGATAGGTTAGCACCACCGAG(+CAGTGT)GATCCTGGAGATATGTTAGCACTTGCACGGA NGG 10 8 17 8 24 0 100 5 10
    240605230033_hj0p AGAAATCTGGGTAAAGAGGAGAGAGATATGGG(+CAGTGT)GTACCCTCCTCCCCTCACTGCTTGTGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605230031_qqv0 GAGATGAGGGCACAAGCAGTGAGGGGAGGAGG(+CAGTGT)GTACCCCATATCTCTCTCCTCTTTACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240605230030_2mcn GTGAGGTGGGAGAAGTGTGTATACCCCTCCTG(+CAGTGT)GATCCTGAATGTTTCTGCCTTTCCTTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605230029_gg89 CCCTCCCCCAAAGGAAAGGCAGAAACATTCAG(+CAGTGT)GATCCAGGAGGGGTATACACACTTCTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605230027_m3g0 ATCACAGACAATACAGCTTTTAACAAGCTGAG(+CAGTGT)GTACCTTTCTTTTTTTAGTGCTAATGATTCA NGG 10 8 17 8 24 0 100 5 10
    240605230026_jteu TAGGTTGAATCATTAGCACTAAAAAAAGAAAG(+CAGTGT)GTACCTCAGCTTGTTAAAAGCTGTATTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605230023_ct69 GTTAGCACCTAGAGGCCGAATCTAACGTCCCG(+CAGTGT)GATCCCATGCCTTGACACCCACAGCCCAATC NGG 10 8 17 8 24 0 100 5 10
    240605230021_ntee TCTAAGATTGGGCTGTGGGTGTCAAGGCATGG(+CAGTGT)GATCCGGGACGTTAGATTCGGCCTCTAGGTG NGG 10 8 17 8 24 0 100 5 10