Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605225944_twpy GCCATCACCAAAGGGTGCAGCCACAGGGCTGG(+CAGTGT)GTACCTGCATGCTTCATCTCCACCTGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605225943_y37f AGGATGCCTTCAGGTGGAGATGAAGCATGCAG(+CAGTGT)GTACCCAGCCCTGTGGCTGCACCCTTTGGTG NGG 10 8 17 8 24 0 100 5 10
    240605225940_9oj1 GTTTCCAGCTTTAGTACAGATGGCTGCCTGGG(+CAGTGT)GATCCCAGAAGCCTTCCGGACCCTACTTCCT NGG 10 8 17 8 24 0 100 5 10
    240605225938_e5cg CCCAAAGGAAGTAGGGTCCGGAAGGCTTCTGG(+CAGTGT)GATCCCCAGGCAGCCATCTGTACTAAAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605225936_mio1 TGGCCTACGATGTTCTTCAGTCCCTGTTCTGG(+CAGTGT)GATCCTGGGGCTTATCTTGGGCACAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605225933_20b7 TCTTAGCCTTCTGTGCCCAAGATAAGCCCCAG(+CAGTGT)GATCCCAGAACAGGGACTGAAGAACATCGTA NGG 10 8 17 8 24 0 100 5 10
    240605225932_ccgy TTGGCTCTGAGACCTGGAGCGCGTGCTCAGTG(+CAGTGT)GATCCCCGGGCAGGTGGGCCTCTGTGGCTCA NGG 10 8 17 8 24 0 100 5 10
    240605225928_6v7u GGGTCTGAGCCACAGAGGCCCACCTGCCCGGG(+CAGTGT)GATCCACTGAGCACGCGCTCCAGGTCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605225927_6hxo TCCCACCTGAGGGTCTGGCGTGTTTCCATCTG(+CAGTGT)GATCCCTTTCTGCAGAGGTTCACAGGCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605225925_k0yx TAGACATGGGCCTGTGAACCTCTGCAGAAAGG(+CAGTGT)GATCCAGATGGAAACACGCCAGACCCTCAGG NGG 10 8 17 8 24 0 100 5 10