Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230447_o1fa AGGGTCAGGGCTGTTGTGAGGACTAACTCCGG(+CAGTGT)GTACCCTTTTCCTGTTCCTTTCCCCCTGTTC NGG 10 8 17 8 24 0 100 5 10
    240605230445_odoe GCCTGGAACAGGGGGAAAGGAACAGGAAAAGG(+CAGTGT)GTACCCGGAGTTAGTCCTCACAACAGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605230442_a3wn CCACATTTGGAAGGATGGAAGTCTAAGTGGGG(+CAGTGT)GTACCTGAAGGAGCTCTGTCTCTGTCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605230441_ratt ATTGGGCAGAGACAGAGACAGAGCTCCTTCAG(+CAGTGT)GTACCCCCACTTAGACTTCCATCCTTCCAAA NGG 10 8 17 8 24 0 100 5 10
    240605230439_37ck AGTGCTTAAGAGTGCTTACTGTACTTCCAAAG(+CAGTGT)GATCCAAGTCTGCTCCCATGCAGGGCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240605230438_phgl CTGCATGGGAGCCCTGCATGGGAGCAGACTTG(+CAGTGT)GATCCTTTGGAAGTACAGTAAGCACTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240605230437_ixvi CCCTTCCCGACCGGGAGGTAATAAAATAGTTG(+CAGTGT)GATCCAGTTCTGACTTGCTAGGTGCTCTAGG NGG 10 8 17 8 24 0 100 5 10
    240605230435_utr8 CCCCGCCTAGAGCACCTAGCAAGTCAGAACTG(+CAGTGT)GATCCAACTATTTTATTACCTCCCGGTCGGG NGG 10 8 17 8 24 0 100 5 10
    240605230433_5lxs TCCTCCAAGCTCTCAGGAGGCAGAGGCAGGTG(+CAGTGT)GATCCCTGTGAGTTTGAGGCCAGGGCTACAC NGG 10 8 17 8 24 0 100 5 10
    240605230431_pdfu TCACTGTGTAGCCCTGGCCTCAAACTCACAGG(+CAGTGT)GATCCACCTGCCTCTGCCTCCTGAGAGCTTG NGG 10 8 17 8 24 0 100 5 10