Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230732_qzvp GTTCAGTCCCCAGGTCAGAAGGGGAGAGTGTG(+CAGTGT)GATCCTGACATGGTTCTATACTCTTCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605230731_c2lz ATTACCCCCAGAAGAGTATAGAACCATGTCAG(+CAGTGT)GATCCACACTCTCCCCTTCTGACCTGGGGAC NGG 10 8 17 8 24 0 100 5 10
    240605230730_bw90 GTGACCATTTTTTACACAACCATACTGGAAAG(+CAGTGT)GATCCAGTACCTTTTTTTTTTTAGTGTGATT NGG 10 8 17 8 24 0 100 5 10
    240605230728_hk5d TCTGAAATCACACTAAAAAAAAAAAGGTACTG(+CAGTGT)GATCCTTTCCAGTATGGTTGTGTAAAAAATG NGG 10 8 17 8 24 0 100 5 10
    240605230726_dlgq TCTTTTCATCAAAAACCTTATCATTTCCTATG(+CAGTGT)GTACCATTGTCTGTCTCCAGAGTGTGTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605230725_jzs4 CAGATTTTGACACACTCTGGAGACAGACAATG(+CAGTGT)GTACCATAGGAAATGATAAGGTTTTTGATGA NGG 10 8 17 8 24 0 100 5 10
    240605230723_59wu ACTGTGTAGCCTAGGTTGATATCAAATCAATG(+CAGTGT)GTACCCTCAGCACACATACCAGTTCAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605230722_dsht TAACTGAGGCTGAACTGGTATGTGTGCTGAGG(+CAGTGT)GTACCATTGATTTGATATCAACCTAGGCTAC NGG 10 8 17 8 24 0 100 5 10
    240605230721_4j2n TAGACAAGCCACTTGTTGTATCTGAGTTTTAG(+CAGTGT)GTACCAGAGGGGAAATACTGCCTGATCGATT NGG 10 8 17 8 24 0 100 5 10
    240605230719_wqn3 ATAAAAATCGATCAGGCAGTATTTCCCCTCTG(+CAGTGT)GTACCTAAAACTCAGATACAACAAGTGGCTT NGG 10 8 17 8 24 0 100 5 10