Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605230838_wad5 AAGTTGATAGAAATTGTCTTATCCATATTCTG(+CAGTGT)GTACCTGAGTAATTCTGCCATTTATTAGATG NGG 10 8 17 8 24 0 100 5 10
    240605230837_vf4a CCATGCATCTAATAAATGGCAGAATTACTCAG(+CAGTGT)GTACCAGAATATGGATAAGACAATTTCTATC NGG 10 8 17 8 24 0 100 5 10
    240605230834_hsyf CCCAGTTAAGTATCTCACAACTGACTCCAAGG(+CAGTGT)GATCCAACAGAATTCTGGCATCTGAGTGCAC NGG 10 8 17 8 24 0 100 5 10
    240605230833_53xw TGCAGGTGCACTCAGATGCCAGAATTCTGTTG(+CAGTGT)GATCCCTTGGAGTCAGTTGTGAGATACTTAA NGG 10 8 17 8 24 0 100 5 10
    240605230832_136l TTTCCAAATTGTATACAAGTCGATTCATTAAG(+CAGTGT)GATCCAACTAGTAACATCATTCATTTAATGC NGG 10 8 17 8 24 0 100 5 10
    240605230830_ergc ACAAAGCATTAAATGAATGATGTTACTAGTTG(+CAGTGT)GATCCTTAATGAATCGACTTGTATACAATTT NGG 10 8 17 8 24 0 100 5 10
    240605230829_1yno GATCAGTTGAGAGCCTTCTGATAACACTGGAG(+CAGTGT)GATCCTGGTCTACAGATGTAGCCAATCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605230827_iuk6 AAGCATCCAGATTGGCTACATCTGTAGACCAG(+CAGTGT)GATCCTCCAGTGTTATCAGAAGGCTCTCAAC NGG 10 8 17 8 24 0 100 5 10
    240605230826_tcq3 AGAACCAGTGAGTTAAGTAGCCTGGGAACTGG(+CAGTGT)GTACCATTTGAGTCAAGCCTTGAAACATGGG NGG 10 8 17 8 24 0 100 5 10
    240605230824_m2ii CCACACCCATGTTTCAAGGCTTGACTCAAATG(+CAGTGT)GTACCCAGTTCCCAGGCTACTTAACTCACTG NGG 10 8 17 8 24 0 100 5 10